摘要
目的探讨肝癌患者热消融治疗对调节性T淋巴细胞（Tregs）的影响及其提高机体抗肿瘤免疫作用的相关机制。方法选择2009年12月至2010年12月行微波消融治疗的肝癌患者36例（观察组），并选择同期健康体检者20例作为对照组。采用流式细胞仪检测Tregs；采用酶联免疫吸附法检测白细胞介素（IL）．10，比较两组外周血Tregs比例和IL．10水平。结果观察组外周血Tregs比例治疗后1周较治疗前明显降低[（5．07±0．82）％比（11．44±2．74）％]（P〈0．01），与对照组的（2．45±0．67）％比较差异均有统计学意义（P〈0．01）。观察组外周血IL-10水平治疗后1周较治疗前明显降低[（25．47±5．43）mg／L比（41．66±9．24）mg／L]（P〈0．01），与对照组的（14．32±3．64）mg／L比较差异有统计学意义（P〈0．01）。结论肝癌患者存在Tregs比例和IL-10水平的高表达，减低Tregs比例以及下调IL-10水平可能是热消融治疗提高机体抗肿瘤免疫作用的主要机制。
Objective To investigate the changes of regulatory T cells （Tregs） in hepatocellular carcinoma（HCC） patients with thermal ablation therapy （TAT）, and to explore the mechanisms of immunityenhancement induced by TAT. Methods A total of 36 patients with HCC undergoing TAT （observation group） were enrolled in this study from December 2009 to December 2010. Another 20 healthy person were selected as control group. The percent of Tregs was detected by flow cytometry analysis. The level of serum IL-10 was measured with ELISA. Results Before treatment, the percent of Tregs in TAT group was significantly higher than that in control group [ （ 11.44 ± 2.74）% vs. （2.45 ± 0.67）% ] （P 〈 0.01 ）. After treatment, the percent of Tregs in TAT group decreased significantly [ （ 11.44 ± 2.74）% vs. （5.07 ±0.82）% ] （P 〈 0.01 ）. The level of serum IL-10 in TAT group [ （41.66 ±9.24） mg/L] was significantly higher than that in control group [ （ 14.32 ±3.64） mg/L] （P 〈 0.01 ）. After treatment, the level of serum IL-10 in TAT group decreased significantly[ （41.66 ± 9.24） mg/L vs. （25.47 ±5.43） mg/L] （P 〈 0.01 ）. Conclusion TAT can enhance immunity in HCC patients, and which may accomplish by down regulation of Treg and IL-10.
出处
《中国医师进修杂志》
2012年第35期23-25,共3页
Chinese Journal of Postgraduates of Medicine
关键词
癌
肝细胞
T淋巴细胞
消融治疗
Carcinoma, hepatocellular
T-lymphocytes
Thermal ablation treatment
