摘要
为了提高鱼骨钙的提取效果,利用高压脉冲电场从淡水鱼骨中辅助提取鱼骨钙制备高钙鱼骨水解液。单因素试验和二次通用旋转组合设计试验表明,脉冲数、电场强度、柠檬酸与苹果酸比对鱼骨钙的提取率影响显著;建立鱼骨钙提取率与各影响因子间关系的回归数学模型;确定了高压脉冲电场辅助提取鱼骨钙的最佳工艺参数为电场强度为25kV/cm、脉冲数为8个、柠檬酸与苹果酸的质量比为1:1g/g、酸料比为1:1g/g、水料比为12:1mL/g,此时处理4g鱼骨粉所得的鱼骨水解液中钙提取率达84.2%。与超声波辅助提取鱼骨钙对比试验表明,高压脉冲电场辅助提取鱼骨钙的得率较超声波辅助提取提高了17%,且其耗时短。该研究可为高压脉冲电场辅助提取鱼骨钙深入研究及其高钙鱼骨口服液的研制提供借鉴参考。
In order to improve the efficiency of fishbone calcium extraction, the high intensity pulsed electric fields （PEF） technique was used for assisted hydrolyzing the fishbone. The single factor experiment and second general revolving combination design results show that the pulse number, the electrical field strength, the mass ratio of citric acid and malic acid are the significant factors on the extraction efficiency of fishbone. With analysis of the regression mathematical model of extraction efficiency and the impact factor; the optimal combination of parameters was found that pulse number was 8, electrical field strength was 25 kV/cm, mass ratio of citric acid and malic acid in mix-acid was 1：1 g/g, acid charge proportion was 1：1 g/g, and water-powder ratio was 12：1 mL/g. Under the optimized condition, the extraction rate of 4 g fishbone power hydrolysate was up to 84.2%. The results show that comparing with ultrasonic technique, high intensity pulse electric fields is less time-consuming with higher extraction effeciency. The study can provide a reference for further studies of calcium fishbone extraction by PEF technique and the development of high calcium fishbone oral liquid.
出处
《农业工程学报》
EI
CAS
CSCD
北大核心
2012年第23期265-270,共6页
Transactions of the Chinese Society of Agricultural Engineering
基金
长春市科技支撑项目(09KZ22)
吉林大学基本科研业务费项目(450060445128)
关键词
提取
工艺
优化
鱼骨
高电压脉冲电场(PEF)
钙
extraction, processing, optimization, fishbone, high intensity pulsed electric fields （PEF）, calcium
