摘要
以3个不同生境黄檗菌根为研究对象,采用酸性品红染色法分析黄檗根系AM真菌侵染情况;利用PCR-DGGE技术并结合DGGE图谱分析、DNA测序及系统发育分析,研究黄檗菌根AM真菌菌群组成及多样性,揭示不同生境黄檗AM真菌菌群动态变化规律,为黄檗菌根功能菌群的研究奠定基础。结果表明:不同生境黄檗根系与AM真菌均能形成良好的共生关系,并且侵染率、DGGE图谱条带丰度和优势度存在差异。城市人工林地区菌根的侵染率、丰度、优势度、多样性指数均高于天然次生林和天然原始林,且天然原始林地区菌根各项检测指标最低。DGGE条带测序和系统发育分析显示:全部序列可分为4类菌群,即球囊霉属、盾孢囊霉属、多孢囊霉属和肉盘菌科。城市人工林、天然次生林和天然原始林黄檗根系样品中最具优势的AM真菌均为Glomus属。
In this study, the mycorrhizae of Phellodendron amurense in three different habitats were used as the research objects. The root samples were investigated by means of acid fuchsin stain after they were lysed in alkaline solution. Then, the community composition and diversity of different AM fungi species in samples were analyzed using PCR-DGGE. DGGE results were analyzed in conjunct（on with DGGE profiles, DNA sequencing and phylogenetics to investigate the pattern of dynamic variation of the AM fungi of Phellodendron amurense in three different habitats, lay the foundation for the study for functional flora in mycorrhizal of P. amurense. The results showed that the roots and AM fungi of P. amurense in different habitats were able to form symbiosis. Distinct differences were detected in infection rate, DGGE band abundance and dominancy. The infection rate, abundance, dominancy and diversity index of the mycorrhizae in urban plantation areas were significantly higher than that in natural secondary forest and natural primary forest. All the detection indexes of the mycorrhizae in natural primary forest was lowest. Sequencing and phylogenetic analysis revealed these sequences could be divided in four groups： Glomus, Scutellospora, Diversispora and Hyponectria buxi. Glomus fungi were the dominant species in the roots of P. amurense in the urban plantation, natural secondary forest and natural primary forest.
出处
《林业科学》
EI
CAS
CSCD
北大核心
2012年第9期99-107,共9页
Scientia Silvae Sinicae
基金
黑龙江省博士后基金项目(LBH-Z05013)
黑龙江大学高层次人才支持计划项目(生态修复团队Hdtd2010-12)
黑龙江大学博士启动基金项目
