摘要
目的观察以壳聚糖衍生物(NSC)为载体,与蛇床子素(OST)共价结合后形成的具有亲骨性和亲水性的新型化合物—蛇床子素骨靶向药物(NSC-OST)对体外培养大鼠成骨细胞(Osteoblast,OB)增殖功能的影响,探讨其防治骨质疏松的作用机制。方法取新生大鼠颅盖骨进行OB分离、培养,采用碱性磷酸酶(ALP)染色及茜素红染色法鉴定OB;随机将OB分为5组,即空白对照组及终浓度为10-7 mmol/L、10-6 mmol/L、10-5 mmol/L、10-4 mmol/L NSC-OST实验组,分别加入不同浓度的NSC-OST作用不同时间,用MTT法检测成骨细胞增殖情况。结果体外分离培养的细胞具有典型成骨细胞形态学及生物学活性,碱性磷酸酶、茜素红染色均呈阳性结果;作用24 h、48 h时,终浓度为10-5mmol/L NSC-OST可显著促进OB增殖(P<0.05),作用72 h,终浓度为10-6mmol/L和10-5mmol/LNSC-OST可显著促进OB增殖(P<0.05);终浓度为10-4mmol/L NSC-OST具有明显抑制OB增殖的作用(P<0.01)。结论 NSC-OST的水溶性良好,理化性质稳定,适当浓度的NSC-OST可促进成骨细胞增殖,有望成为有效抗骨质疏松作用的新型药物。
Objective To observe the effect of bone-targeted osthole （NSC-OST） , a new type of chemical compound with chitosan as carrier characterized by bone-specific and hydrophilicity, on cell proliferation of rat osteoblasts in vitro, and to explore the mechanism of NSC-OST in the prevention and treatment of osteoporosis. Methods The osteoblasts were isolated and cultured from the calvaria of new-born rats. The cells were identified with alkaline phosphatase staining and Alizarin red staining. The osteoblasts were randomly divided into 5 groups： blank group, 10^-7 mmol/L NSC-OST group, 10^-6 mmol/L NSC-OST group, 10^-5 mmol/L NSC-OST group, and 10^-4 mmol/L NSC-OST group. Rat osteoblasts cultured in vitro were additioned with a series of concentrations of NSC-OST for different time. MTT method was used to test the proliferation of osteoblasts. Results The ceils cultured in vitro showed representative morphological and biological characteristics of osteoblasts. Alkaline phosphatase and Alizarin red staining presented positive results. After 24h and 48h of the treatment, the proliferation of osteoblusts was promoted in the presence of 10-5 mmol/L NSC-OST （P 〈0. 05）. The proliferation of osteoblasts was promoted in the presence of 10^-6 mmol/L or 10^ -5 mmol/L NSC-OST in 72 h of the treatment （P 〈 0.05 ）. The proliferation of osteoblasts was obviously inhibited in the presence of 10 ^-4 mmol/L NSC-OST （P 〈 0. 01 ）. Conclusion NSC-OST is good of water-solubility and stable physicoehemical property. NSC-OST with certain concentrations promotes osteoblast proliferation, which is potent of a new type of anti-osteoporotic drug.
出处
《中国骨质疏松杂志》
CAS
CSCD
北大核心
2012年第9期781-784,共4页
Chinese Journal of Osteoporosis
基金
江苏省中医药局科技项目(LZ09016)
南京中医药大学基础研究与重点培育专项基金项目(09XJC04)
