摘要
[目的]研究大孔吸附树脂分离纯化桃叶珊瑚苷的工艺,为进一步精制洒金桃叶珊瑚中桃叶珊瑚苷奠定基础。[方法]以桃叶珊瑚苷为指标成分,用HPLC法测定桃叶珊瑚苷的含量,并优选大孔吸附树脂分离纯化桃叶珊瑚苷的最优工艺。[结果]S-8大孔吸附树脂分离纯化桃叶珊瑚苷的最优条件为:上样液浓度2.76 mg/ml,上样液流速2 BV/h,上样量9.20 mg/g,洗脱溶媒为浓度15%乙醇,洗脱流速2 BV/h,洗脱剂用量3 BV。[结论]S-8大孔吸附树脂对桃叶珊瑚苷分离纯化效果良好,产品纯度为48.04%,回收率为82.30%。
[Objective] To study the separation and purification process of aucubin with macroporous resin,so as to lay foundation for preparing aucubin from Aucuba japonica Variegata.[Method] Taking aucubin as index,the concentration of aucubin was measured by HPLC to optimize the best separation and purification conditions of aucubin with macroporous resin.[Result] The optimum conditions of S-8 macroporous resin were as follows： sample concentration of 2.76 mg/ml,sample flow velocity of 2 BV/h,sample capacity of 9.20 mg/g,elutant of 15% ethanol aqueous,elution flow rate of 2 BV/h,and total quantity of eluant of 3 BV.[Conclusion] It has good effect to separate aucubin by S-8 macroporous resin,the content and recovery rate of aucubin were 48.04% and 82.30%
出处
《安徽农业科学》
CAS
2012年第28期13787-13789,共3页
Journal of Anhui Agricultural Sciences
基金
四川省科技支撑项目(2008SZ0009)
中央高校基本科研专项(NO:SWJTU11ZT25)
