摘要
采用聚丙烯酰胺凝胶电泳方法对19份石榴品种的过氧化物酶(POD)同工酶酶谱进行分析,并利用系统聚类的方法,研究了19个品种间的亲缘关系。结果表明:19个品种的POD同工酶酶谱多态性较高,不同品种的酶带数目、相对迁移率及酶活性均有差异;19个石榴品种的POD同工酶酶谱共呈现12条酶带,其中1条酶带为所有种质共有的酶带;Rf为0.216的谱带为大青皮酸品种的特征谱带,Rf为0.113的酶带为大马牙甜石榴的特征谱带。POD同工酶聚类分析结果在阈值0.60处将19个品种分为5类,类群间遗传差异较大。
The peroxidase （POD） isozyme of 19 pomegranates germplasm cultivars were determined by polyarcylamide gel electrophoresis （PAGE） and the genetic relationship were studied with clustering analysis method. The results showed that a better polymorphism was observed in POD enzyme. The number, the activity and Rf of POD enzyme were difference among 19 pomegranates. 12 enzyme bands were detected and there was a common locus for total experiment material in the zymogram patterns; the two specific loeuses that Rf was 0. 216 and 0. 113 were obtained for ＇Daqingpisuan＇ cultivar and ＇Damayatian＇ cultivar respectively. Furthemore, the clustering analysis indicated 19 pomegranates were divided into five groups based on 0. 60 of similarity coefficient, which indicated that the genetic difference between groups was significant.
出处
《北方园艺》
CAS
北大核心
2012年第19期124-127,共4页
Northern Horticulture
基金
枣庄学院博士科研启动基金资助项目(YSY08003)
关键词
石榴
种质资源
POD同工酶
聚类分析
亲缘关系
Punica granatum L. （pomegranates）
germplasm
POD isozyme
cluster analysis
genetic relationship
