摘要
目的:探讨细胞核增殖指数(Ki-67)、丝氨酸/苏氨酸蛋白激酶(pAKT)在胃癌组织中的表达及与胃癌侵袭转移和复发等生物学行为的关系。方法:收集有完整临床病理资料、经手术及术后病理学确诊的60例胃癌患者作为观察组,并以20例癌旁异型增生胃黏膜组织和20例活检正常胃黏膜组织作为对照组,应用免疫组织化学SP法检测Ki-67、pAKT的表达,用CD31标记血管计数MVD,比较:1)Ki-67、pAKT的表达在胃癌、癌旁组织和正常胃黏膜组织中的表达差异;2)与肿瘤分化程度的关系;3)与癌组织浸润深度和转移的关系。结果:观察组中Ki-67(73.3%,44/60)、pAKT(75.0%,48/64)的表达明显高于对照组癌旁异型组织(35.0%,7/20)和正常黏膜组织(10.0%,2/20),差异有统计学意义,χ2=9.538,P=0.002;χ2=24.621,P=0.000。Ki-67、pAKT在高级别与低级别胃癌组织中的表达差异有统计学意义,P<0.05。Ki-67、pAKT在进展期胃癌显著上调,与早期癌表达差异有统计学意义,P<0.05。Ki-67、pAKT与癌组织淋巴结转移有关,P<0.05。结论:Ki-67、pAKT异常表达与胃癌的发生、发展密切相关,在胃癌组织中表达上调,促进了肿瘤形成、发展、侵袭和转移,Ki-67、pAKT检测对于判断胃癌生物学行为有一定的价值,可作为临床参考指标。
OBJECTIVE： To investigate the expression of Ki-67 and pAKT in gastric carcinoma and their relationship with invasion and metastasis. METHODS： Sixty cases of gastric carcinoma with complete clinical datas and diagnosed by pathology intraoperatively and postoperatively were collected as the observation group. The expressions of Ki-67, pAKT were measured by immunohistochemical UltraSensitiveTM SP method staining in 60 cases of primary gastric carcinoma, 20 cases of dysplasia tissue closely adjacent to carcinomas, and 20 cases of normal gastric mucosa specimens. RESULTS： The expressions of Ki-67 and pAKT in gastric cancer were （73.3 % ,44/60） and（75.0 %,48/64） repectively, the expressions of Ki-67 and pAKT in gastric tissues obviously was higher than the expression in adjacent tissue organizations（35.0%, 7/10） and normalmucosaltissue （10.0%, 2/20,X2=9.538,P=0.002;x2=24. 621,P=0. 000）. The expression of Ki- 67, pAKT was positively correlated to the infiltration of gastric and lymph node metastasis （P〈0.05）. CONCLUSIONS： The occurrence and development of gastric carcinoma may be closely related to the expression of Ki-67, pAKT. The ab normal expression of Ki-67 and pAKT play an important role in the genesis and development of gastric carcinoma and may be responsible for the enhanced activity in tumor-induced neovascularization, invasion and metastasis. Ki 67, pAKT have positively expressive correlation and may serve as valuable indicators of biological behavior of gastric carcinoma.
出处
《中华肿瘤防治杂志》
CAS
北大核心
2012年第15期1156-1158,共3页
Chinese Journal of Cancer Prevention and Treatment
