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PTEN在喉癌中的表达及其与DNA含量的关系

The Relationship Between the Expression of PTEN and the DNA Content In Laryngeal Carcinoma
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摘要 目的:探讨喉癌中PTEN的表达及其与DNA含量的关系。方法:采用免疫组化S-P法和流式细胞术,检测PTEN基因蛋白在60例喉鳞状细胞癌、15例喉乳头状瘤及10例正常黏膜的表达及DNA含量。结果:①PTEN蛋白表达定位于细胞质,PTEN蛋白阴性占18.3%(11/60),表达减低者占30%(18/60),阳性表达者占51.7%(31/60),PTEN与组织分化程度、临床分期相关。②喉癌的DI值高于对照组(P<0.05)。PTEN阳性的喉癌细胞DNA含量明显低于阴性组(P<0.05)。结论:PTEN在喉癌的发生发展中起着重要的作用,它的检测对喉癌的判定、预后和进一步治疗提供有效的依据。 Objective：To investigate the relationship between the expression of PTEN and the DNA content in laryngeal carcinoma.Methods：Expression of PTEN protein was measured with immunohistochemistry method and the DNA quantitative was measured with flow cytometry in 60 cases of laryngeal squamous cell carcinoma,15 cases of larynx papilloma and 10 cases of normal laryngeal mucosa.Results：①PTEN,being show in the cytoplasm,was negative in 18.3 %,reduced in 30% and positive in 51.7%.PTEN expression level was correlated with differentiation degree and clinical stages.②The DNA index of laryngeal carcinoma group was higher than that of other groups（P〈0.05）.The DNA index of laryngeal carcinoma whose expression of PTEN protein was positive was significantly lower than that of negative.Conclusion：This experiment suggests that PTEN may play certain roles in the oncogengsis and progression of laryngeal carcinoma.Detection of the gene prducts has positive offects on early diagnosis,malignancy determination prognosis and treatment of laryngeal carcinoma.
作者 赵辉 彭诗东
机构地区 内蒙古自治区医院耳鼻喉科 内蒙古医学院附属医院耳鼻喉科
出处 《内蒙古医学杂志》 2009年第11期1298-1300,共3页 Inner Mongolia Medical Journal
关键词 喉鳞状细胞癌 PTEN CYCLIND1 免疫组化 流式细胞术 Laryngeal squamous carcinoma PTEN CYCLINDI Immunohistochemistry Flow cytometry
分类号 R739.65 [医药卫生—肿瘤]
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参考文献11

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内蒙古医学杂志
2009年 第11期

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