摘要
分别采用Sevage法、三氯乙酸-正丁醇(TCA-NBA)法、酶-Sevage法以及酶-TCA-NBA法对粒毛盘菌YM281胞外多糖进行脱蛋白。结果表明:用酶-TCA-NBA法对粒毛盘菌YM281胞外多糖脱蛋白的效果最佳,蛋白脱除率为51.57%,且此时多糖损失率(3.46%)为最低;进一步采用均匀设计优化该方法的脱蛋白条件,所得最优脱蛋白条件为:粒毛盘菌YM281多糖溶液20mL、木瓜蛋白酶溶液11mL、温度42.2℃、pH 4、酶解时间3.5h;除去变性蛋白后,再利用TCA-NBA法脱蛋白2次即可;采用所得的最优方法和条件脱蛋白,其蛋白脱除率高达72.3%,多糖损失率仅为2.93%。
Sevage method, TCA-NBA （trichloroacetic acid-N-butyl alcohol） method, enzyme-Sevage method and enzyme-TCA-NBA method were used to remove protein from extracellular polysaccharide of Lachnum YM281, respectively. The results showed that the method of enzyme-TCA-NBA was the most effective, by which the protein-removal rate and the polysaccharide-loss rate were 51.57% and 3.46%, respectively. Then the uniform design was employed to optimize the conditions of this method. As a result, the optimal conditions of the enzyme-TCA-NBA method were as follows： polysaccharide solution of Lachnum YM281 of 20 mL, papain solution of 11 mL, pH 4, zymolysis time of 3.5 h at 42.2 ℃ ; after removing the denatured protein, TCA-NBA method was used remove protein twice. Under this optimal method and conditions, the protein-removal rate reached up to 72.3% and the polysaccharideloss rate was only 2.93 %.
出处
《浙江大学学报（农业与生命科学版）》
CAS
CSCD
北大核心
2012年第4期519-523,共5页
Journal of Zhejiang University:Agriculture and Life Sciences
基金
国家大学生创新实验计划资助项目(091035952)
安徽高校省级自然科学研究重大资助项目(kj2010zd10)
关键词
粒毛盘菌
胞外多糖
脱蛋白
条件优化
Lachnum sp.
extracellular polysaccharide
deproteinization
condition optimization
