摘要
为提供非洲菊化学诱变的基础材料以及相应的组培操作技术,采用非洲菊品种‘琳达’的幼嫩花托为外植体进行组织培养研究,外植体最佳消毒时间为流水冲洗1 h,75%的酒精浸泡30 s,0.1%的HgCl2灭菌12 min;诱导产生愈伤组织的最适培养基为:MS+6-BA 5.0 mg/L+NAA 0.5 mg/L+蔗糖3%(w/v)+琼脂粉7%(w/v);增殖培养基配方MS+6-BA 1.0 mg/L+NAA 0.1 mg/L+蔗糖3%(w/v)+琼脂粉7%(w/v)为最适配方;生根壮苗培养基以1/2MS+NAA0.1 mg/L+蔗糖2%+琼脂粉7%为最佳。试验显示以花托为外植体较为容易进行表面灭菌,诱导愈伤组织并分化丛生芽,从而快速建立离体快繁体系。
To provide the basis of the materials for Gerbera’s chemical inducement;and the corresponding set of technique.Using gerbera varieties young receptacle of‘Linda’as explants to tissue culture.the best sterilization time on explants were washed for one hour and sterilized with 75% ethyl alcohol for 30 s,0.1% HgCl2 for 12 minutes,the calluses introduction medium for in vitro culture was MS（murashige and skoog） with 6-BA（5.0 mg/L） ＋ NAA（0.5 mg/L） ＋ sucrose（3%） ＋ agar（7%）,the multiplication medium was MS with 6-BA（1.0 mg/L）＋NAA（0.1mg/L） ＋ sucrose（3%）＋agar（7%）,the rooting medium was 1/2MS with NAA（0.1 mg/L）＋ sucrose（2%）＋agar（7%）.From the experiments,it was evident that young receptacle as explants was more easy to surface sterilization,callus induction and multiple shoot clumps,then established rapid propagation system of Gerbera.
出处
《农学学报》
2012年第3期31-35,共5页
Journal of Agriculture
基金
浙江省级创新项目"特殊空间绿化材料新优草花的引种与评价"(2011R50034-06)
关键词
非洲菊
组织培养
花托
Gerbera
Tissue Culture
Receptacle
