摘要
[Objective] The aim was to clone triosephosphate isomerase （TPI） gene from Apis mellifera, and predict the properties of TPI protein with bioinformatic meth- ods. [Method] The TPI gene was firstly cloned by in silico cloning based on the ex- pressed sequence tags （ESTs） from Unigene of NCBI. Some characters of the TPI protein including hydrophobicity or hydrophilicity, isoelectric point （pl） and secondary structure were analyzed and predicted by the tools of bioinformatics. [Result] The TPI gene from A. mellifera was 1 768 bp in full length and it contained a complete ORF which encoded 247 amino acids; the pl of TPI protein was 8.515; the TPI protein was a member of ~13-fold family. [Conclusion] The in silico cloning based on the expressed sequence tags is a efficient method in practice, and this study will provide more references for further study on A. mellifera at molecular level.
[目的]克隆蜜蜂(Apis mellifera)TPI基因,并进行生物信息学预测。[方法]利用电子克隆方法获得蜜蜂磷酸甘油醛异构酶(Triosephosphate isomerase,TPI)基因,并采用生物信息学方法对该基因编码蛋白的等电点、疏水性/亲水性、二级结构等进行了预测。[结果]蜜蜂TPI基因全长为1768bp,具有完整的开放阅读框架(ORF),所编码蛋白的等电点为8.515。二级结构预测表明TPI蛋白属于α/β蛋白。[结论]利用蜜蜂表达序列标签数据库(EST)电子克隆蜜蜂新基因的研究工作有一定的现实意义,为进一步在分子水平研究蜜蜂提供更多的参考。
基金
Supported by Scientific Research Fund of Changzhi University (2010111)~~
