摘要
目的观察芪药消渴胶囊对高糖诱导肾小球系膜细胞(GMCs)增殖的抑制作用。方法通过GMCs体外培养技术,采用含药血清药理学的方法,制备不同浓度芪药消渴胶囊的含药血清,筛选最佳葡萄糖浓度,在葡萄糖的诱导下,通过四甲基偶氮唑蓝(MTT)比色法检测并观察不同时间、不同浓度下GMCs增殖情况。结果在不同时间点,空白组细胞生长最为缓慢。与空白组比较,在相同培养条件下,0.4 g/L葡萄糖组及0.5 g/L葡萄糖组在48 h、72 h对GMCs增殖有刺激作用(P<0.05,P<0.01),0.6 g/L葡萄糖组0.7 g/L葡萄糖组、0.8 g/L葡萄糖组在24 h、48 h、72 h对GMCs增殖有明显刺激作用(P<0.05,P<0.01),其中0.6 g/L葡萄糖组在48 h、72 h对GMCs增殖刺激作用较强(P<0.01)。提示0.6 g/L葡萄糖为GMCs增殖的最佳刺激浓度。与空白对照组比较,高糖组细胞生长迅速(P<0.05,P<0.01);与高糖组比较,芪药消渴胶囊含药血清各组细胞在48 h、72 h生长缓慢(P<0.05),提示芪药消渴胶囊对高糖诱导的GMCs增殖的抑制作用。结论芪药消渴胶囊具有抑制GMCs增殖作用。
Objective To observe the inhibition of Qiyaoxiaoke capsule on the glomerular mesangial cell（GMCs） proliferation induced by high glucose.Methods Different concentration serum was prepared by serum pharmacological approach of containing drug.Optimum concentration of glucose was selected by mesangial cells in vitro culture techniques.The glomerular mesangial cell proliferation under different times and different concentrations were observed by MTT.Results The cell growth in blank group is the slowest at different time points.Compared with the control group,in the same culture conditions,GMCs proliferation stimulation in 0.4 g/L glucose group and 0.5 g/L glucose group at 48 h,72 h were stronger（P0.05,P0.01）,GMCs proliferation stimulating effect in 0.6 g/L glucose group,0.7 g/L glucose group and 0.8 g/L glucose group at 24 h,48 h,72 h were stronger（P0.05,P0.01）,GMCs proliferation stimulating effect in 0.6 g/L glucose at 48 h,72h were the strongest（P0.01）.GMCs proliferation in high glucose group was more rapid than that in blank control group（P0.05,P0.01）.Compared with the high glucose group,GMCs proliferation in each group of containing Qiyaoxiaoke capsule serum at 48 h,72 h was slow（P0.05）.Conclusion Qiyaoxiaoke capsule has inhibition on the glomerular mesangial cell proliferation.
出处
《河北中医》
2012年第3期434-436,共3页
Hebei Journal of Traditional Chinese Medicine
基金
陕西省2008年"13115"科技创新工程重大科技产业化计划项目(编号:2008ZDCY-25)
关键词
肾小球膜
药物作用
中药
胶囊
糖尿病肾病
血清
Glomerularmembrane
Drug action
Traditional Chinese drug
Capsule
Diabetic nephropathy
Serum
