摘要
目的:观察芒果苷对哮喘小鼠气道炎症的影响,探讨芒果苷平喘的作用机制。方法:将72只4～5周SPF级BALB/c雌性小鼠随机分为正常对照组、模型对照组、地塞米松阳性对照组(0.001 25 g·kg-1)、芒果苷高、中、低剂量组(0.4,0.2,0.1 g·kg-1)。采用卵白蛋白(OVA)致敏和激发构建小鼠哮喘模型,收集支气管肺泡灌洗液(BALF)进行细胞计数及分类;肺组织病理切片HE染色观察炎性细胞浸润情况;采用ELISA法检测小鼠血清中OVA特异性IgE(OVA-sIgE)和BALF中白三烯C4(LTC4),前列腺素D2(PGD2)的含量。结果:芒果苷高、中、低剂量组BALF中嗜酸性粒细胞(EOS)比例由模型组(6.57±1.44)%降低为(1.63±0.43,2.72±0.83,4.11±1.08)%(P<0.01～P<0.05);芒果苷高、中剂量组BALF中白细胞总数由模型组(83.25±10.19)×105/mL减少为(25.66±6.16,53.38±9.19)×105/mL(P<0.01);芒果苷各剂量均可不同程度的改善肺组织的病理学改变,减轻气道炎症;芒果苷高、中、低剂量组血清中OVA-sIgE含量由模型组(0.26±0.04)ng·mL-1降低为(0.10±0.04,0.12±0.02,0.18±0.03)ng·mL-1(P<0.01);芒果苷高剂量组BALF中PGD2水平由模型组(74.36±22.72)ng·mL-1降低为(60.30±12.19)ng·mL-1(P<0.05),而芒果苷中、低剂量组对PGD2的降低无统计学差异;芒果苷各剂量对LTC4水平有降低趋势。结论:芒果苷可通过减少EOS浸润、小鼠体内OVA-sIgE和PGD2含量,抑制哮喘小鼠的气道炎症,在治疗哮喘方面有潜在的应用前景。
Objective： To investigate the inhibitory effect and mechanism of mangiferin on the airway inflammation in BALB/c mice model of asthma.Method： Seventy-two BALB/c mice,aged 4-5 weeks,were randomly divided into 6 groups： normal control group,model control group,dexamethasone group（0.001 25 g·kg-1）,mangiferin high-dose group（0.4 g·kg-1）,mangiferin middle-dose group（0.2 g·kg-1） and mangiferin low-dose group（0.1 g·kg-1）.The mice were sensitized and challenged with ovalbumin（OVA） to establish a murine model of asthma.Bronchoalveolar lavage fluid（BALF） was collected for total and differential cell count.HE staining was used to observe airway inflammation in lung tissue.Quantification of leukotriene C4（LTC4）,prostaglandin D2（PGD2） in BALF and OVA special immunoglobulin E（OVA-sIgE） in serum were detected by ELISA.Result：The number of eosinophil（EOS） in BALF,compared with model group（6.57±1.44）%,could obviously be reduced by three mangiferin groups（1.63±0.43,2.72±0.83,4.11±1.08）%（P0.01-P0.05）.The total number of white cells in BALF,compared with model group（83.25±10.19）×105/mL,could be cut down by mangiferin high-dose and middle-dose group（25.66±6.16,53.38±9.19） ×105/mL（P0.01）;HE staining showed that three mangiferin groups could improve,to some extent,the histological changes of lung and bronchus and reduce airway inflammation.The level of OVA-sIgE in serum,compared with the model group（0.26±0.04） ng·mL-1,could significantly be decreased by three mangiferin groups（0.10±0.04,0.12±0.02,0.18±0.03） ng·mL-1（P0.01）.And the level of PGD2 in BALF,compared with model group（74.36±22.72） ng·mL-1,could be reduced by mangiferin high-dose group（60.30±12.19） ng·mL-1（P0.05）.For the level of LTC4,there was a reduce tendency in every mangiferin group,but no statistical significance.Conclusion： Mangiferin,through reducing the number of EOS,decreasing the level of OVA-sIgE and PGD2 in mice,could obviously inhibit the airway inflammation in mice model of asthma,which promises to be a potential drug in the treatment of allergic asthma.
出处
《中国实验方剂学杂志》
CAS
北大核心
2012年第9期187-190,共4页
Chinese Journal of Experimental Traditional Medical Formulae
基金
2009年广西科技厅自治区重点实验室培育项目(09-007-06)
