摘要
目的观察碱性成纤维细胞生长因子(b-FGF)在不同肝纤维化分期(S)肝组织中表达的差异性及其与临床检测常用的肝功能指标、肝纤维化指标、HBV DNA复制水平以及肝硬化Child-Pugh分级之间的关系。方法选取122例HBV引起的慢性肝炎及肝硬化患者,空腹静脉血检测:(1)肝功能指标:ALT、TBil、Alb;(2)肝纤维化指标:透明质酸(HA)、Ⅲ型前胶原(PCⅢ)、层粘连蛋白(LN)、Ⅳ型胶原(CⅣ);(3)HBV DNA复制水平;(4)对临床诊断肝硬化患者计算其Child-Pugh评分。行肝脏穿刺活检术后,经病理作纤维化分期(S1～S4)。免疫组织化学法测定肝组织中b-FGF在不同病理分期的表达及定位特点,半定量分析法计算b-FGF值,观察b-FGF与肝功能指标、肝纤维化指标、HBV DNA水平以及肝硬化Child-Pugh分级之间的关系。结果 (1)肝纤维化S1～S4期肝组织中b-FGF的表达部位及水平不同;(2)肝纤维化S1～S4期肝细胞中b-FGF的表达与肝纤维化分期(S1～S4)呈显著正相关(r=0.542,P<0.01);(3)不同肝纤维化分期中b-FGF的表达差异与患者ALT、TBil及Alb进行比较,其差异无统计学意义(P>0.05),不同肝纤维化分期患者,进行HBV DNA检测,其差异无统计学意义(P>0.05);(4)不同肝纤维化分期中,b-FGF与血清纤维化指标HA、PCⅢ、LN、CⅣ之间呈正相关;(5)b-FGF的表达与临床Child-Pugh分级无明显相关性。结论 b-FGF的表达与肝脏纤维化程度、肝纤维化指标有正相关关系,与患者ALT、TBil、Alb、Child-Pugh评分、及HBV DNA水平无关。
Objective To observe expressions of b-FGF in different stage on the hepatic fibrosis and to discuss the relationship with indexes of hepatic function,indexes of hepatic fibrosis,hepatic cirrhosis Child-Pugh classification,HBV DNA replication.Methods 122 cases of HBV-induced chronic hepatitis and cirrhosis were selected.Delect hepatic function（ALT,TBil,Alb）,indexes of liver fibrosis： hyaluronic acid（HA）,Ⅲ procollagen（PC Ⅲ）,laminin（LN）,Ⅳ collagen（CⅣ）,HBV DNA replication were tested and hepatic cirrhosis Child-Pugh classification were judged.the pathological diagnosis and different stages of the hepatic fibrosis of 122 patients with chronic hepatitis were determined by liver biopsy.b-FGF Value was calculated,the relationship of b-FGF with indexes of hepatic function,indexes of hepatic fibrosis,HBV DNA replication,hepatic cirrhosis Child-Pugh classification was observed.Results 1.There were some difference of expressions of b-FGF in different parts and levels of liver fibrosis S1-S4 stage.2.The expressions of b-FGF were gradually increased with the development of the hepatic fibrosis（S1-S4） and positive correlation was determined（r=0.542,P0.01）.3.In the different processes of the hepatic fibrosis（S1-S4） the expressions of b-FGF with ALT,TBil,ALB,HBV DNA replication were not statistically significant（P0.05）;4.In the different processes of the hepatic fibrosis（S1-S4） the expressions of b-FGF with HA,PC Ⅲ,LN,CⅣ were positively correlated.5.The expressions of b-FGF and Child-Pugh classification had no correlation.Conclusion The expressions of b-FGF have a positive correlation with the level of hepatic fibrosis and indexes of hepatic fibrosis and there is no correlation of expressions of b-FGF with ALT,TBil,Alb,Child-Pugh score and HBV-DNA replication.
出处
《临床肝胆病杂志》
CAS
2012年第4期281-284,共4页
Journal of Clinical Hepatology
基金
中央高校基本科研业务费专项资金资助(lzujbky-2009-150)
