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Roles of Smad3 and Smad7 in rat pancreatic stellate cells activated by transforming growth factor-beta 1 被引量：13

Roles of Smad3 and Smad7 in rat pancreatic stellate cells activated by transforming growth factor-beta 1
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摘要 BACKGROUND: Pancreatic stellate cells (PSCs) play a major role in promoting pancreatic fibrosis. Transforming growth factor beta 1 (TGF-beta 1) is a critical mediator of this process. This study aimed to determine the expression of the Smad3 and Smad7 genes in the process of PSC activation, and explore the mechanisms of chronic pancreatitis. METHODS: The expressions of Smad3 and Smad7 in PSCs before and after TGF-beta 1 treatment were detected by reverse transcription-polymerase chain reaction and Western blotting analysis. Smad3 expression was detected in PSCs after treatment with 5 ng/ml of TGF-beta 1 for 24 hours. RESULTS: Smad7 expression was decreased in TGF-beta 1 -activated PSCs (P<0.05) in a dose-dependent manner. When TGF-beta 1 concentration reached 10 ng/ml, the expression of p-Smad3, Smad3, and Smad7 was inhibited (P<0.05). CONCLUSIONS: TGF-beta 1 promotes the expression of Smad3 and inhibits the expression of Smad7 during the activation of PSCs. In contrast, high-dose TGF-beta 1 downregulates the expression of Smad3 in completely activated PSCs. BACKGROUND: Pancreatic stellate cells (PSCs) play a major role in promoting pancreatic fibrosis. Transforming growth factor beta 1 (TGF-beta 1) is a critical mediator of this process. This study aimed to determine the expression of the Smad3 and Smad7 genes in the process of PSC activation, and explore the mechanisms of chronic pancreatitis. METHODS: The expressions of Smad3 and Smad7 in PSCs before and after TGF-beta 1 treatment were detected by reverse transcription-polymerase chain reaction and Western blotting analysis. Smad3 expression was detected in PSCs after treatment with 5 ng/ml of TGF-beta 1 for 24 hours. RESULTS: Smad7 expression was decreased in TGF-beta 1 -activated PSCs (P<0.05) in a dose-dependent manner. When TGF-beta 1 concentration reached 10 ng/ml, the expression of p-Smad3, Smad3, and Smad7 was inhibited (P<0.05). CONCLUSIONS: TGF-beta 1 promotes the expression of Smad3 and inhibits the expression of Smad7 during the activation of PSCs. In contrast, high-dose TGF-beta 1 downregulates the expression of Smad3 in completely activated PSCs.
作者 Qian, Zhu-Yin Peng, Quan Zhang, Zheng-Wei Thou, Long-An Miao, Yi
机构地区 Nanjing Med Univ
出处 《Hepatobiliary & Pancreatic Diseases International》 SCIE CAS 2010年第5期531-536,共6页 国际肝胆胰疾病杂志（英文版）
基金 supported by grants from the Natural Science Foundation of Jiangsu Province,China (No. BK2006241) the Foundation for Talents in Six Fields of Jiangsu Province (No. 07-B-038)
关键词 pancreatic stellate cell transforming growth factor beta 1 chronic pancreatitis SMAD3 SMAD7 pancreatic stellate cell transforming growth factor beta 1 chronic pancreatitis Smad3 Smad7
分类号 R735.9 [医药卫生—肿瘤]
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参考文献23

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二级参考文献15

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Hepatobiliary & Pancreatic Diseases International
2010年 第5期

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