摘要
目的:研究壳寡糖对乳酸杆菌体外生长的影响。方法:将乳酸杆菌MRS培养基中的葡萄糖替换为壳寡糖及向原培养基中加入适量的壳寡糖,通过测定OD值比较乳酸杆菌的生长状况。结果:以壳寡糖完全代替葡萄糖的培养基中乳酸杆菌的生长状况不如MRS培养基,而少量壳寡糖与葡萄糖协同的培养条件使乳酸杆菌的生长适应期明显减短,促进其生长。结论:乳酸杆菌对壳寡糖的利用不如对葡萄糖的利用,1g/l壳寡糖与葡萄糖协同作用时可明显缩短乳酸杆菌的生长适应期,促进细菌生长。
Objective： To investigate the effect of Chito-Oligosaccharide （COS）on the growth of Lactobacillus in vitro. Methods： Replace glucose with COS in Lactobacillus medium and apply moderate COS to the original medium. Lactobacillus growth was detected by measuring its OD value. Results： Lactobacillus growth in the original medium is better than that in the medium of COS replaced for glucose, and the lag phase in the medium adding a little COS is shorter than the original medium, promote the multiplication of Lactobac- illus in vitro. Conclusion： Glucose is better than COS for the growth of Lactobacillus. The synergistic action of COS and glucose can shorten the growth lag phase and promote the multiplication of Lactobacillus with 1 g/1 COS.
出处
《现代生物医学进展》
CAS
2012年第5期849-851,839,共4页
Progress in Modern Biomedicine
