摘要
采用免疫亲和柱净化、在线电化学衍生化高效液相色谱法测定了花生中黄曲霉毒素(AFT)B1,B2,G1和G2。以体积分数为80%的甲醇提取样品中的AFT,经免疫亲和柱净化洗脱后,以KobraCell装置在线衍生,反相HPLC分离定量。4种毒素的分离在13min内完成,检出限均达到0.1μgkg。5次测定花生样品的RSD值为9.2%～15%;样品添加标样0.5～9.0μgkg,回收率为74.8%～97.3%。
A method for the determination of aflatoxin B 1,B 2,G 1 and G 2 in peanut by high performance liquid chromatography using immunoaffinity column clean up and on line electrochemical derivatization was developed. Sample was extracted with 80% methanol and the extract was passed through the IAC. Aflatoxins were retained by the monoclonal in the IAC and eluted with actonitrile. AFT B 1 and G 1 were derivatized with on line electrochemical equipment Kobra Cell. The four toxins can be separated in 13 minutes and the detection limit was 0.1 μg/kg for each toxin.The method has been applied to the determination of peanut sample. The relative standard deviation was 9.2% 15% and the average recovery of AFT spiked at 0.5 9.0 μg/kg levels was in the range of 74.8% 97.3%.
出处
《色谱》
CAS
CSCD
北大核心
2000年第1期82-84,共3页
Chinese Journal of Chromatography
关键词
高效液相色谱法
电化学衍生
黄曲霉毒素
花生
high performance liquid chromatography
electrochemical derivatization
immunoaffinity column
aflatoxin
peanut
