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地方性氟中毒患者外周血淋巴细胞COLIXA3基因的表达 被引量：1

COLIXA3 gene expression of peripheral blood lymphocyte in patients with endemic fluorosis
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摘要 目的探讨氟暴露人群中，氟中毒与COLIXA3（the a3 chain of collagen Ⅸ）mRNA表达水平的关系，观察在地方性氟中毒的发病机制中COLIXA3基因所起的作用。方法2009年，选取新疆生产建设兵团农七师123团和128团确诊的轻度氟中毒患者12人为病例组，生活在氟中毒病区10年以上的6例健康人作为内对照组．生活在非氟中毒病区10年以上的6例健康人作为外对照组，采集静脉血，分离淋巴细胞，采用SYBR Green Ⅰ嵌合荧光法进行实时定量PCR，对氟骨症患者和对照组淋巴细胞中COLIXA3mRNA进行相对表达量的检测。结果病例组、内对照组、外对照组COLIXA3基因的相对表达量分别为2．16±0．62、1．06±0．09、1．05±0．12。病例组COLIXA3表达明显上调，高于内对照组和外对照组（P均〈0．05），内对照组与外对照组组间比较差异无统计学意义（P〉0．05）。结论过量氟可促进患者外周血淋巴细胞中COLIXA3mRNA的表达．达到对照组的2倍以上，可能有生物标志物意义。 Objective To study the relationship between expression of a3 chain of collagen IX（COLIXA3） mRNA in the population exposed to fluorine and fluorosis, in order to reveal the role of COLIXA3 gene in the pathogenesis of endemic fluorosis. Methods Twelve cases of mild drinking water-born skeletal fluorosis were selected as case groups in Regiment 123 and 128 of Xinjiang Production and Construction Corps Seven Division, 6 cases of healthy people living in fluorosis areas for more than 10 years as a internal control group and 6 heathly cases living in non-fluorosis areas for more than 10 years as a external control group. The expression of COLIXA3 mRNA of peripheral blood lymphocyte of skeletal fluorosis patients and control groups were determined by using SYBR Green I chimeric fluorescent method for real-time quantitative PCR. Results The results of the relative expression of COLIXA3 mRNA of case group, internal control group and external control group were 2.16± 0.62, 1.06 ±0.09 and 1.05 ±0.12, respectively. The COLIXA3 expression in case group was significantly higher than that of the internal control group and the external control group （all P 〈 0.05）, while the difference of COLIXA3 expression between the internal control group and the external control group was not significantly different （P 〉 0.05）. Conclusions Fluorine contributes to the expression of COLIXA3 mRNA in peripheral blood lymphocyte, and the expression is up to 2 times higher than that of the control groups, meaning potential hiomarkers.
作者 唐莉 王立杰 张亚楼 白生宾 钟近洁 张跃新 刘开泰
机构地区 新疆医科大学第一附属医院感染科 基础医学院组织与胚胎学教研室 新疆兵团疾病预防控制中心疾控科 中国疾病预防控制中心教育培训处
出处 《中国地方病学杂志》 CAS CSCD 北大核心 2012年第2期144-146,共3页 Chinese Jouranl of Endemiology
基金 国家自然科学基金（30860249、81160331）
关键词 氟化物中毒 Ⅸ型胶原a3链基因 反转录聚合酶链反应 Fluoride poisoning The a3 chain of collagen IX genes Reverse transcriptase polymerasechain reaction
分类号 R599 [医药卫生—内科学]
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  • 1J．萨姆布鲁克 金冬雁译.分子克隆实验指南，第2版[M].北京:科学出版社,1992.304-308.
  • 2Saamanen AM, Salminen, de Crombrugghe, et al. Osteoarthritis-like lesions in transgenic mice harboring a small deletion mutation in type I collagen gene[J]. Osteoarthritis Cart, 2000,8:248-257.
  • 3Faissler R, Schnegelsberg PNJ, Dausman J, et al. Mice lacking at (IX) collagen develop noninfalmmatory degenerative joint disease [J]. Proc Natl Acad Sci USA, 1994,91:5070-5074.
  • 4Nakata K, Onok, Miyazaki J, et al. Osteoarthritis associated with mild chondrodysplasia in transgenic mice expressing alpha 1 (IX) collagen chains with a central deletion [J]. Proc Natl Acad Sci USA, 1993,90:2870-2874.
  • 5Spayde EC, Joshi AP, Wilcox WR, et al. Exon skipping mutation in the COL9A2 gene in a family with multiple epiphyseal dysplasla[J]. Matr Biol, 2000,19:121-128.
  • 6Matshi Y, Yashui N, Kawabata H, et al. Anovel type X collagen gene mutation (G595R) associated with Schmid-type metaphyseal chondrodysplasia [J]. J Hum Genet, 2000,45:105-108.
  • 7Chan D, Cole WG, Rogers JG, et al. Type X collagen multimer assembly in vitro is prevented by a gly 618 to val mutation in the alphal( X ) NC1 domain resulting in schmid metaphyseal chondrodyaplasia [J]. J Biol Chem, 1995, 270 (9) :4558-4562.
  • 8Mclntosh I, Abott MH, Warman MI, et al. Additional mutations of type X collagen confirm COL10A1 as the schmid metaphyseal chondrodysplasia locus [J]. Hum Mol Genet,1994,3(2) :303-307.
  • 9Seegmiller RE, Ryder V, Sheldon H. Comparison of two collagen mutant mouse lines that serve as models of earlyonset osteoarthritis in human chondrodyspalsia [J]. Osteoarthritis Cart, 2001,9 (suppl B) :S15.
  • 10Li SW, Takanosu M, Arita M, et al. Targeted disruption of COLllA2 produces a mild cartilage phenotype in transgenic mice: comparison with the human disorder otospondyl omega epiphyseal dysplasia[J]. Dev Dyn, 2001,222:141-152.

共引文献24

同被引文献70

  • 1李崇斌,孙发,肖跃海,石家齐,谷江,刘鲜林,常傲霜,谢春.燃煤型氟中毒对大鼠睾丸超微结构的影响[J].贵州医药,2011,35(2):99-103. 被引量：8
  • 2陈诚,逯宜,王书彦,李晓红.不同剂量氟中毒大鼠剩余牙槽骨骨改建的研究[J].西安交通大学学报（医学版）,2012,33(1):110-113. 被引量：3
  • 3Duan X,Mao Y,Wen X,et al.Excess fluoride interferes with chloride-channel-dependent endocytosis in ameloblasts[J].J Dent Res,2011,90(2):175-180.
  • 4Wei W,Gao Y,Wang C,et al.Excessive fluoride induces endoplasmic reticulum stress and interferes enamel proteinases secretion[J].Environ Toxicol,2013,28(6):332-341.
  • 5Yang T,Zhang Y,Zheng D,et al.High-fluoride promoted phagocytosis-induced apoptosis in a matured ameloblast-like cell line[J].Arch Oral Biol,2014,60(1):84-90.
  • 6Li P,Xue Y,Zhang W,et al.Sodium fluoride induces apoptosis in odontoblasts via a JNK-dependent mechanism[J].Toxicology,2013,308:138-145.
  • 7刘康康,霍亮亮,裴俊瑞,等.PI3K-Akt信号转导通路在氟促成骨细胞代谢过程中的作用研究[A].第七次全国地方病学术会议论文集[C].哈尔滨:中国地方病学杂志编辑部,2011:164.
  • 8Huo L,Liu K,Pei J,et al.Fluoride promotes viability and differentiation of osteoblast-like Saos-2 cells via BMP/Smads signaling pathway[J].Biol Trace Elem Res,2013,155(1):142-149.
  • 9徐辉,张金铭,吕鹏,等.PERK信号因子在染氟成骨细胞样细胞基因表达的变化[A].第七次全国地方病学术会议论文集[C].哈尔滨:中国地方病学杂志编辑部,2011:158-161.
  • 10Yu J,Gao Y,Sun D.Effect of fluoride and low versus high levels of dietary calcium on mRNA expression of osteoprotegerin and osteoprotegerin ligand in the bone of rats[J].Biol Trace Elem Res,2013,152(3):387-395.

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中国地方病学杂志
2012年 第2期

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