摘要
目的:本研究旨在证实孟加拉国传统药用植物千根草甲醇提取物的抗高血糖及镇痛作用。方法:实验分为独立的两个部分。在研究千根草甲醇提取物抗高血糖作用的实验中,36只小鼠被分为6组,每组6只,分别给予口服不同剂量的千根草甲醇提取物、降血糖药格列本脲及10 mL/kg 1%Tween 80溶剂,1 h后,予各组小鼠口服2 g/kg葡萄糖,2 h后测取所有小鼠的血糖水平并进行比对。为观察千根草甲醇提取物的镇痛作用,另外42只小鼠被分为7组,每组6只,分别给予各组小鼠口服10 mL/kg 1%Tween 80溶剂、不同剂量千根草甲醇提取物,或200和400 mg/kg镇痛药阿司匹林1 h后,向所有小鼠腹腔内注射10 mL/kg 1%醋酸诱发扭体反应。诱发扭体反应5 min后,每只小鼠观察10 min,记录扭体次数。结果:分别口服50、100、200和400 mg/kg千根草甲醇提取物的4组小鼠与对照组相比血糖水平显著降低(P<0.05)。当小鼠口服400 mg/kg千根草甲醇提取物时,血糖水平下降最多。口服10 mg/kg格列本脲的小鼠血糖水平下降48.6%。与对照组相比,甲醇提取物同样被证实具有显著的镇痛作用(P<0.05)。当服用剂量在400 mg/kg时,小鼠扭体次数抑制率达40.9%,而口服200 mg/kg镇痛药阿司匹林时,小鼠扭体次数抑制率为49.0%。结论:孟加拉国民间草药千根草在治疗糖尿病与疼痛方面有显著作用,其甲醇提取物抗高血糖及镇痛的作用值得进一步研究,以便开发出以此植物为基础的新型化合物与有效药。
OBJECTIVE： To study the antihyperglycemic and antinociceptive activities of methanolic extract of the whole plant of Euphorbia thymifolia L., a plant used in folk medicine of Bangladesh for treatment of diabetes and pain. METHODS. Antihyperglycemic activity studies were conducted in glucoseloaded mice by oral glucose tolerance tests. Mice were given various doses of the extract, followed by glucose （2 g/kg body weight）, h after administration of the extract. Serum glucose levels were measured 2 h after glucose administration. Antinociceptive activity studies were conducted in intraperitoneally acetic acidinjected mice through measurement of reductions in abdominal writhing times caused by acetic acidinduced gastric pain. Following a period of 1 h after oral administration of various doses of the extract, all mice received intraperitoneal injection of 1% acetic acid at a dose of 10 mL/kg body weight. To ensure bioavailability of acetic acid, a period of 5 min was given to each animal following which period the number of writhings was counted for 10 min. RESULTS. The extract caused a significant dose-dependent reduction in serum glucose levels in mice, when administered at doses of 50, 100, 200 and 400 mg/kg body weight as compared to the control animals （P^0.05）. The highest reduction of serum glucose （60. 5%） was observed at a dose of 400 mg/kg. In comparison, a standard antihyperglycemic drug glibenclamide, when administered at a dose of 10 mg/kg body weight, lowered serum glucose levels by 48.6%. The extract also demonstrated a significant dosedependent antinociceptive activity compared to control animals （P〈0.05）. At a dose of 400 mg/kg body weight, the number of abdominal writhings was inhibited by 40.9% as compared to 49.0% inhibition obtained with a standard antinociceptive drug aspirin, administered at a dose of 200 mg/kgbody weight. CONCLUSION. The significant antihyperglycemic and antinociceptive activities demonstrated by the extract validate the use of E. thymifolia in folk medicine of Bangladesh for treatment of diabetes and pain, and merit further scientific studies leading to discovery of efficacious drugs.
出处
《中西医结合学报》
CAS
2012年第2期228-232,共5页
Journal of Chinese Integrative Medicine
基金
supported by internal funding from the University of Development Alternative,Bangladesh
关键词
大戟属
植物提取物
降血糖药
镇痛药
小鼠
Euphorbia
plant extracts
hypoglycemic agents
analgesics
mice
