摘要
针对油藏中的产甲烷菌检测繁琐、费时的问题，研究荧光原位杂交技术检测产甲烷菌，并结合产甲烷菌数量、低分子有机酸和甲烷气体体积分数的变化，分析模拟油藏条件下微生物驱油过程中产甲烷菌的变化规律。在模拟油藏条件下，培养30d后，产甲烷菌的数量逐渐升高；培养50d后，总菌数增加；前期由其他微生物代谢产生的乙酸等产物作为产甲烷菌所需的代谢底物被消耗，随着培养时间的延长，产甲烷菌的生长代谢又促进了其他微生物的生长。应用荧光原位杂交技术可将检测时间缩短到1d以内，快速、准确地检测模拟油藏条件下产甲烷菌的变化，为明确各种微生物的生长代谢对驱油效果的贡献提供依据。
In light of the problem of thee ie0mplicated and tedious detection of the methanogen in oil reservoirs, the detecting technique of in-situ hybridized＇fluorescence is developed. Integrating with the changes of methanogen amount, low molecular weight organic acid and the volume fractions of the methane, the change laws of the methanogen in the course of microbial oil flooding are analyzed under the conditions of simulated oil reservoirs. In the ＇above case, after culturing 30 days, the amount of the methanogen increases gradually; after 50 days, the total bacterial number rises ; ahead of time, the generated acetic acid and the other products by the metabolism of other microorganisms are consumed by methanogen-needed metabolism substrata. With the time of the culture, the growth and metabolism of the methanogen also promote the growth of the other microorganisms. With the help of the technique, the detected time can be reduced within one day ; this rapid and accurate detection of the methanogen changes under the conditions of simulated oil reservoirs can provide an evidence for the clear contribution of various microbes＇ growth and metabolism on the oil flooded results.
出处
《大庆石油地质与开发》
CAS
CSCD
北大核心
2012年第1期145-149,共5页
Petroleum Geology & Oilfield Development in Daqing
基金
国家科技重大专项“胜利油田薄互层低渗透油田开发示范工程”（2011ZX05051）资助.
关键词
微生物驱油
产甲烷菌
微生物采油技术
荧光原位杂交
寡核苷酸探针
microbial oil flooding
methanogen
microbial oil producing technique
fluorescence in situ hybridization
oligonucleotide probe
