摘要
针对胜利油田孤岛中一区Ng3地层水的性质设计激活剂配方,在物理模拟条件下有效激活内源微生物,同时将硫酸盐还原菌浓度控制在50个/mL以下。微生物经激活后代谢活跃,产乙酸达346 mg/L,产出气体中甲烷含量5%。通过变性梯度凝胶电泳及其条带测序,定性分析了激活后内源微生物群落中的15个不同内源菌属,其中以嗜热厌氧菌为主,部分种属代谢产生的低分子有机酸、CO2和H2等,被其它细菌或产甲烷古菌作为代谢底物而利用,这些微生物组成了物理模拟实验条件下的完整代谢体系。
Activating nutrients was designed for Zhongyi block in Shengli Oil Field according to the character of stratal water, which could stimulate indigenous microorganisms effectively, and keep Sulfate Reducing Bacteria under 50 cellsomL-1, where acetate and methane produced by microbe were 346 mg.L-1 and 5%, respectively. 15 different genuses of indigenous microorganisms were detected by Denaturing Gradient Gel Electrophoresis and sequencing, most of which were thermophilic anaerobes. Some bacteria could produce low molecular weight organic acids, CO2 and H2, which could be used as substrates by other bacteria and archaea, all of these microorganisms form an entire ecology system under physical simulation conditions.
出处
《石油钻采工艺》
CAS
CSCD
北大核心
2010年第1期89-93,共5页
Oil Drilling & Production Technology
基金
胜利油田博士后项目"油藏驱油微生物群落调控及变化规律研究"(编号:YKB0707)
关键词
油藏
微生物提高采收率
内源微生物
物理模拟实验
群落结构
oil reservoir
microbial enhanced oil recovery
indigenous microorganism
physical simulation
microbial community structure
