摘要
目的评价不同剂量利多卡因对脓毒症大鼠小肠高迁移率组蛋白B1（HMGBl）表达的影响。方法成年雄性Wistar大鼠50只，体重200～250g，采用随机数字表法，将大鼠随机分为5组（n=10）：假手术组（S组）不行盲肠结扎穿孑L术；脓毒症组（Sep组）采用盲肠结扎穿孔术法制备脓毒症模型；不同剂量利多卡因组（L1-3组）于术后即刻、1、2h时分别腹腔注射利多卡因5、10、20mg／kg，S组和Sep组分别给予等容量生理盐水。于术后24、48h时各组随机取5只大鼠处死取小肠组织，光镜下观察小肠组织病理学形态，采用PCR技术检测HMGBlmRNA的表达，ELISA法测定二胺氧化酶（DAO）活性。结果与S组比较，Sep组和L1-3组各时点大鼠HMGBlmRNA表达上调，DAO活性降低（P〈0．05）；与Sep组比较，L1-3组大鼠HMGBlmRNA表达下调，DAO活性升高（P〈0．05）；各时点L1-3组大鼠HMGBlmRNA表达依次下调，DAO活性依次升高（P〈0．05）。L1-3组小肠组织病理学损伤较Sep组减轻。结论利多卡因可呈剂量依赖性减轻脓毒症大鼠小肠损伤，其机制可能与抑制HMGBl表达上调有关。
Objective To investigate the effect of different doses of lidocaine on expression of the high mobility group box 1 protein （HMGB1） in small intestine in septic rats. Methods Fifty adult male Wistar rats weighing 200-250 g were randomly divided into 5 groups （ n = 10 each） ： sham operation group （group S）, sepsis group（group Sep ） and different doses of lidocaine group （group L1-3 ）. Group S were not applied cecal ligation and puncture （CLP）. Sepsis intestinal damage model was performed in group Sep by CLP. Group L1-3 were given intra- peritoneally lidoealne in a dose of 5,10 and 20 mg/kg at immediately, 1 and 2 h after CLP, respectively. Isometric normal saline was given intraperitoneally in group S and group Sep. The small intestine tissues were taken at 24 and 48 h after CLP. The small intestine morphology was observed with optical microscope. The expression of the HMGB1 mRNA were examined by PCR and the activity of diamine oxidase （DAO） were determined by ELISA. Results Compared with group S, the expression of HMGB1 mRNA was increased and the activity of DAO decreased in group Sep and groups L1-3 at 24 and 48 h after CLP （ P 〈 0.05 ）. Compared with group Sep, the expression of HMGB1 mRNA was decreased and the activity of DAO increased in a dose-dependent manner in groups LI.3 （ P 〈 0.05）, The injury of pathology of small intestine was slighter in groups L1-3 than in group Sep. Conclusion Lido- caine can reduce samll intestine injury through inhibiting HMGB1 expression in septic rats by a dose-dependent maimer.
出处
《中华麻醉学杂志》
CAS
CSCD
北大核心
2011年第9期1133-1135,共3页
Chinese Journal of Anesthesiology
基金
山东大学自然科学基金（Y2007C115,2009ZRBl4031,JQ200808）
