摘要
目的:研究妇科再造丸(FKW)对雌、孕激素负荷大鼠子宫病理形态学的影响。方法:将60只雌性SD大鼠随机分为6组:正常对照组、模型组、FKW低、中、高(0.28,0.56,1.12 g.kg-1.d-1)剂量组、桂枝茯苓胶囊组(0.3 g.kg-1.d-1)。除正常对照组外,其余5组肌肉注射己烯雌酚和黄体酮复制大鼠子宫平滑肌瘤样增生模型,从第9周末开始灌胃给予FKW干预,共18周。末次给药后,取大鼠子宫组织HE染色镜下观察其病理形态学改变,同时测定子宫平滑肌厚度。结果:与正常对照组比较,模型组大鼠子宫肌层明显增厚(P<0.01),子宫组织出现肌纤维增生肥大、排列紊乱、穿插及中性粒细胞浸润等病理现象,对上述指标进行半定量统计分析显示差异具有显著性(P<0.01)。FKW能显著减少模型大鼠子宫肌层增厚的程度(P<0.05,P<0.01),改善子宫的病理组织形态学变化(P<0.05,P<0.01)。结论:FKW可以抑制雌、孕激素负荷引起的大鼠子宫平滑肌瘤样增生。
Objective：To study the pathomorphological change of uterus in rats loaded by estradiol and progesterone and the interfering effects of Feke Zaizao pill（FKW）.Method： Sixty female SD rats were divided into 6 groups randomly：the normal group,the model group,the FKW low,moderate,high group（0.28,0.56,1.12 g·kg-1·d-1）,the Guizhi Fuling capsule group（0.3 g·kg-1·d-1）.The rats,except for the normal group,were injected with diethylstilbestrol and progesterone for eighteen weeks to establish uterine leiomyoma-like hyperplasia model.And from the end of the ninth week,FKW was used to interfering with the model rats by gastric perfusion.The uterus tissue was used to processing pathology detection by hematoxylin-eosin staining to observe the pathomorphological changes and the thickness of uterine smooth muscle to study the influence of FKW.Result： Compared with the normal group,the myometrium of rats in the model group incrassation obviously（P0.01）,and the pathologic changes of uterus tissue of rats in the model group,such as smooth muscle fibre hypertrophy、irregular arrangement、interveined and the neutrophil infiltration in the uteri tissue,appeared significant differences which were analyzed by semiquantitative statistic analysis（P0.01）.FKW could minish the degree of myometrium incrassation（P0.05,P0.01）,and improve the pathomorphological changes of uterus of model rats.Conclusion：FKW has the function of restraining and improving the uterine leiomyoma-like hyperplasia in rats,which could be produced by diethylstilbestrol and progesterone loaded.
出处
《中国实验方剂学杂志》
CAS
北大核心
2011年第23期140-144,共5页
Chinese Journal of Experimental Traditional Medical Formulae
基金
贵州省科技厅中药现代化专项资金项目[黔科合中药专字(2003)10号]
