摘要
采用CTAB法和SDS法对通过花粉管通道法导入植酸酶基因(phyA)的玉米植株,进行基因组提取并做了对比,认为SDS法效果更好,进而进行了PCR检测,并对PCR反应体系及反应条件进行优化,得出最适反应条件为57℃、模板含量1μL,检测得到了转基因阳性植株,初步证明外源目的基因已整合到玉米基因组中。
The CTAB method and SDS method were separately used to extract genome from phyA transgenic maize obtained by the pollen-tube pathway.By comparison,the SDS method obtained a better result.The genome was analyzed by PCR method,and the reaction system and reaction conditions were optimized.The optimal reaction condition was 57℃ with 1μL DNA as template.The result of PCR detection showed that positive transgenic plants were obtained and the foreign phyA gene was integrated into maize genome.
出处
《河南农业科学》
CSCD
北大核心
2011年第10期41-43,共3页
Journal of Henan Agricultural Sciences
基金
国家转基因专项(20082X08003-005)
吉林省财政厅项目(200806)
吉林省科技厅成果转化补助项目(20095044)
