摘要
目的建立种植和野生甘西鼠尾草中丹参酮Ⅰ的测定方法并行含量比较。方法借助高效液相法,通过摸索试验条件建立相关试验方法。结果丹参酮Ⅰ进样量在(0.05～3.2)μg/mL范围内与峰面积呈良好的线性关系。回归方程为y=18748.96x+116.71,r=0.9963。回收率为97.75%,种植甘西鼠尾草丹参酮Ⅰ含量1年生为0.090%,2年生为0.156%,3年生为0.192%;野生品种为0.161%。结论所建方法分离度高,重现性好,结果准确可靠,适于甘西鼠尾草中丹参酮Ⅰ的含量测定。种植甘西鼠尾草中丹参酮Ⅰ含量以三年生为高。
Objective To establish the determination method of Tanshinone I and to compare the content in Salvia przewalskii by HPLC.Methods With the methods of HPLC to set up related experimental methods by searching for experimental factors.Results HPLC method exhibited a linear relation of the peak area to the concentration within 0.05~3.2 μg/mL of Tanshinone I.The regression equation was y=18748.96x＋116.71（r=0.9963） and the average recovery was 97.75%,resoectively.The content of Tanshinone I was 0.090% in one year old planting Salvia przewalskii,0.156% in two years old planting Salvia przewalskii and 0.192% in three years old planting Salvia przewalskii.The content of wild varieties was 0.161%.Conclusion The HPLC method is accurate,with a high reproducibility and result is believable.The content of Tanshinone I was highest in three years old planting Salvia przewalskii.
出处
《青海医学院学报》
CAS
2011年第3期196-199,共4页
Journal of Qinghai Medical College
基金
青海大学医学院中青年科技基金项目(2005-ky-11)
