摘要
背景:研究认为Rho激酶可致使神经生长锥塌陷,对神经修复具有抑制作用。目的:观察Rho激酶抑制剂法舒地尔及RNA干预介导的RhoA基因沉默对脊髓损伤大鼠在体神经损伤修复的作用。方法:雄性SD大鼠60只半切法制成脊髓半横断模型,随机等分成对照组、法舒地尔组和RhoA siRNA组。法舒地尔组于腹腔注射10mg/kg法舒地尔,2次/d,连续用药1周;RhoA siRNA干扰组将RhoA siRNA表达质粒注射于大鼠脊髓损伤区。结果与结论:伤后4周,法舒地尔和RhoA siRNA组大鼠后肢运动功能均有明显恢复,可见少量神经轴索样结构,辣根过氧化物酶阳性神经纤维数增多(P<0.05),体感诱发电位的潜伏期明显缩短、波幅显著增强(P<0.05)。提示大鼠脊髓损伤后给予Rho激酶抑制剂法舒地尔及RNA介导的RhoA基因沉默能够促进受损伤的脊髓神经功能恢复。
BACKGROUND：Several studies have demonstrated that Rho kinase can lead to collapse of nerve growth cone and exhibits an inhibitory effect on nerve repair.OBJECTIVE：To investigate the effect of Rho kinase inhibitor Fasudil and RNAi-mediated RhoA gene silencing on nerve repair in a rat model of spinal cord injury.METHODS：Sixty male Sprague-Dawley rats were prepared into spinal cord hemisection and then were randomly divided into control,Fasudil and RhoA siRNA groups.The Fasudil group rats were intraperitoneally injected with 10 mg/kg Fasudil,twice a day,for successive 1 week.The RhoA siRNA group rats were injected with RhoA siRNA expression plasmid into spinal cord injury area.RESULTS AND CONCLUSION：At 4 weeks after injection,the hind limb motor function of the Fasudil and RhoA siRNA group rats was obviously recovered,neuronal axon-like structure was observed,horseradish peroxidase-positive nerve fibers were increased（P 0.05）,somatosensory evoked potential latency was obviously shortened,and amplitude was significantly increased（P 0.05）.These results showed that after spinal cord injury,Rho kinase inhibitor Fasudil and RNAi-mediated RhoA gene silencing can promote the neurofunctional recovery of injured spinal cord.
出处
《中国组织工程研究与临床康复》
CAS
CSCD
北大核心
2011年第33期6147-6151,共5页
Journal of Clinical Rehabilitative Tissue Engineering Research
