摘要
目的:建立一种测定丹酚酸A中有关物质含量的方法。方法:用外标法测定丹酚酸C的含量,采用Diamonsil C18柱(4.6 mm×250 mm,5μm),乙腈-0.2%磷酸水溶液(30∶70)为流动相,流速1 mL.min-1,检测波长285 nm,柱温30℃,以主成分自身对照法控制其他杂质的总含量。结果:丹酚酸C的含量均<1.0%,其他杂质峰面积和<3.0%。结论:该方法专属性强,灵敏度高,简单准确,能有效控制丹酚酸A中有关物质的含量。
Objective：To establish a method for the determination of reference substances in salvianolic acid A. Method： Diamonsil C18 column（4.6 mm×250 mm, 5 μm）, acetonitrile-0.2% phosphoric acid solution（30∶70）as mobile phase, flow rate 1 mL·min-1, detection wavelength 285 nm, column temperature 30 ℃, other relative substances were controlled by main component self-compare method. Result： The content of salvianolic acid C was less than 1.0%, and the others were less than 3.0%.Conclusion：This method is specific, sensitive, simple, accurate, and can effectively detect salvianolic acid C.
出处
《中国实验方剂学杂志》
CAS
北大核心
2011年第19期94-97,共4页
Chinese Journal of Experimental Traditional Medical Formulae
