摘要
目的 用超临界流体萃取法 (SFE)联用毛细管气相色谱法 (CGC)测定丹参药材中丹参酮IIA 的含量。方法 用溶解度参数理论预测丹参酮IIA 的溶解压力 ,再用正交设计法考察温度、改性剂量和动态萃取体积三因素对SFE萃取效率的影响。结果 SFE CGC法测定丹参药材中丹参酮IIA 简便快速 ,结果准确可靠 ,加样回收率为95 3% ,RSD =4 3 % ,n =3。结论 利用SFE CGC法测定丹参药材中丹参酮IIA,可提高分析速度 ,且使用有机溶剂少 ,对环境造成的污染小 ,与超声提取法比较 ,经t检验 ,显著优于超声提取法。
AIM To analyze tanshinone II A in Salvia miltiorrhiza Bunge by supercritical fluid extraction (SFE) coupled with capillary gas chromatography (CGC). METHODS The solution pressure was predicted by solubility parameter theory, the extraction conditions were confirmed by orthogonal design in which temperature, volume of modifier and dynamic extracting volume had been studied in three levels. RESULTS This method is quick, convenient, precise and accurate, added sample recovery is 95 3%, RSD=4 27%. CONCLUSION Compared with the method of ultrasonic extraction, significantly better qualitative and quantitative result could be obtained by SFE CGC and expend less organic solvent then make less pollution.
出处
《药学学报》
CAS
CSCD
北大核心
2001年第1期55-57,共3页
Acta Pharmaceutica Sinica
基金
国家自然科学基金资助项目(39770904)
