摘要
目的:探讨温针治疗肾阳虚证骨关节炎的分子机制,为其疗效提供科学依据。方法:根据肾阳虚证评定量表,选取典型肾阳虚证患者10名给予温针治疗两周,然后以其中疗效最好的4例治疗前后作基因芯片杂交测试,采用SAM基因芯片数据分析软件,筛选显著表达基因作进一步的基因功能分析。结果:4例患者皆为典型肾阳虚证,经温针治疗后效果明显。通过SAM软件筛选到32个显著表达基因,基因功能注释结果提示炎症反应、信号转导类基因显著表达;分子通路主要集中于免疫及信号转导通路。讨论:温针治疗肾阳虚证膝骨关节炎的疗效的分子机制是多方面的,涉及到炎症反应、免疫功能、信号系统等多方面的修复作用。
Objectives： To investigate the molecular mechanisms behind warm needling using Cdna microarray technology,thus providing further scientific evidence for its efficacy.Method： Ten patients with knee OA of kidney-yang deficiency were selected for 2 weeks of warm needling treatment.This treatment involved stimulating the selected acupoints using needles with a burning moxa stick on their handle for 40 minutes per session.Accumulated clinical scores of kidney-yang deficiency were assessed pretreatment and posttreatment using a 40-factor questionnaire of OA with kidney-yang deficiency.Four patients with the best therapeutic efficacy were selected for cDNA microarray testing.The significantly expressed genes were screened through Significance Analysis of Microarray（SAM） for further functionally annotated.Results： 32 significantly expressed genes were screened.GO and PATHWAY annotation results of these genes showed that immune and signal transduction functional genes and pathways significantly expressed.Conclusion： This work demonstrates that the molecular mechanism behind warm needling treatment may be associated with the regulation of immune and signal transduction genes and pathways.
出处
《云南中医学院学报》
2011年第4期4-6,10,共4页
Journal of Yunnan University of Traditional Chinese Medicine
基金
国家自然科学基金资助项目(NO:81072731)
关键词
肾阳虚证
骨关节炎
温针
差异基因表达谱
kidney-yang deficiency
osteoarthritis
warm needing
differential gene expression profile
