摘要
目的:探讨艾灸延缓衰老的可能作用机制。方法:将30只实验大鼠随机分为空白组、模型组、艾灸组,每组10只。皮下注射25%D-半乳糖溶液(125 mg/kg)造成大鼠衰老模型。艾灸组选取双侧"脾俞""肾俞"进行艾灸治疗,每日治疗1次,每次每穴灸5 min,连续治疗6周。采用组织化学染色及多克隆抗体免疫组化技术,结合细胞医学图像分析及病理图文图像分析方法,测定各组大鼠松果体细胞数及HSP 70表达。结果:与空白组相比,模型组大鼠松果体细胞数显著减少(P<0.001)、HSP 70表达显著降低(P<0.01)。与模型组相比,艾灸组大鼠松果体细胞数显著增多(P<0.01)、HSP 70表达显著增加(P<0.05)。结论:艾灸可能通过上调脑区松果体中HSP 70表达、减缓机体衰老过程中松果体细胞减少的速度,在一定程度上维持松果体的正常形态及功能,延缓松果体衰退,从而起到抗衰老的作用。这可能是艾灸延缓机体衰老机制之一。
Objective To study the mechanism of moxibustion in postponing the process of aging. Methods Thirty Wistar rats were equally randomized into control, model and moxibustion groups. The subacute aging model was established by hypodermic injection of 25% D-galactose （125 mg/kg）. Moxibustion was applied to bilateral ＂Shenshu＂ （GV 23） and ＂Pishu＂ （GV 20） once everyday for 6 weeks. After slicing, the pineal gland tissue was stained with HE and insitu hybridization methods respectively for detecting the quantity of pinealocytes and the expression of heat shock protein （HSP 70）. Results Compared with the control group, both the quantity of pinealocytes and the expression of HSP 70 in the pineal grand in the model group were downregulated significantly （P〈0. 001, P〈0.01 ）. Compared with model group, the quantity of pinealocytes and of HSP 70 mRNA in the pineal gland of moxibustion group were upregulated significantly（ P〈0.01, P〈0.05）. Conclusion Moxibustion can suppress aging induced decrease of pinealocyte number and HSP 70 expression in subacute aging rats, which may contribute to its effect in postponing aging.
出处
《针刺研究》
CAS
CSCD
北大核心
2011年第4期258-261,267,共5页
Acupuncture Research
关键词
艾灸
衰老
松果体松果体细胞数
热休克蛋白70
Moxibustion
Aging
Pineal gland
Pinealocytes
Heat shock protein 70 expression
