摘要
对所构建的7种水稻同核异质雄性不育系进行细胞质遗传效应研究,包括主要生长特性和农艺性状、与恢复系测配的影响以及花粉发育的细胞学观察。结果表明,7种同核异质不育系的播始历期为81～83d,剑叶长为25.10～29.40cm,穗长为21.45～24.30cm,株高为80.60～94.13cm,每穗粒数在163.2～222.1。与恢复系辐恢838和绵恢725进行测配,结果表明用绵恢725配组时,各组合的生育期为113～115d,剑叶长和剑叶宽2个性状与用辐恢838配组时类似。最主要的差别在株高、有效穗、每穗粒数、每穗实粒数、结实率和千粒重等几个性状。总体看株高明显下降,每穗粒数和实粒数显著增加,而结实率和千粒重多数不如用辐恢838配组的组合。用7种同核异质雄性不育系混合后与单一恢复系配组,在实践中是可行的。细胞学观察结果表明,同核异质雄性不育系花粉粒败育开始在单核期,92%以上为典败。花粉粒败育时,维管束的结构被彻底破坏。由此推断,所用的同核异质雄性不育系的败育应该同维管束的不正常发育有关,其败育机理应该是相同的。
The cytoplasmic genetic effects,including the growth characteristics and agronomic traits of the seven types of isonuclear alloplasmic male sterile lines（IAMSLs） and of the combinations derived from the IAMSLs and the restorer lines,were analyzed and the cytological study of the pollen development was also carried out.The IAMSLs were 81-83 days in duration from seeding to heading,25.10-29.40 cm in flag leaf length,21.45-24.30 cm in panicle length,80.60 to 94.13 cm in plant height,and 163.2-222.1 in grain number per panicle.Compared with the combinations of the IAMSLs and the restorer line Fuhui 838,the combinations derived from the IAMSLs and Mianhui 725 showed the same tendency in the traits of flag leaf length and width,as well as the growth duration of 113-115 days.The major differences between them were the plant height,effective panicle number,total grain number per panicle,number of filled grains per panicle,seed setting rate and 1000-grain weight.In general,the combinations orignated from Mianhui 725,the total grain number per panicle,number of filled grains per panicle and seed setting rate were gradually increased,but the seed setting rate and the 1000-grain weight were lower than the combinations derived from Fuhui 838.Furthermore,it had been proved that crossing the mixture of seven types of IAMSLs with a single restorer line was feasible in practice.Based on the cytological observation,the cell nucleuses of the seven types of IAMSLs began degradation at the uninucleate stage with an abortion rate above 92%.The anther vasculars were quietly destroyed during the pollen development in the male sterile lines.It was concluded that the sterility of the seven types of IAMSLs was closely related to the abnormal development of vascular tissues and might have the same abortion mechanism in pollen development in these male sterile lines of rice.
出处
《中国水稻科学》
CAS
CSCD
北大核心
2011年第4期370-380,共11页
Chinese Journal of Rice Science
基金
国家自然科学基金资助项目(3097174030821064)
国家973计划资助项目(2007CB108705)
湖北省自然科学基金重点项目(2011002923388)
关键词
水稻
同核异质雄性不育系
农艺性状
微卫星标记
细胞学观察
rice
isonuclear alloplasmic male sterile line
agronomic trait
simple sequence repeat
cytological observation
