摘要
采用反相高效液相色谱法测定了牙膏中的甘草次酸。在 Ｙ Ｗ Ｇ Ｃ１８（４０ ｍ ｍ ｉ．ｄ．×２５０ ｍ ｍ ，１０ μｍ ）色谱柱上，以 Ｖ（甲醇）∶ Ｖ（００１ ｍ ｏｌ／ Ｌ Ｋ Ｈ２ Ｐ Ｏ４）＝ ８５∶１５（ｐ Ｈ ３０）的溶液为流动相，流速为 １０ ｍ Ｌ／ｍ ｉｎ，紫外检测波长为 ２５４ ｎｍ ，室温下检测。甘草次酸用甲醇提取。甘草次酸的平均回收率为 ９９６１％ ～１０１６７％ ，样品测试相对标准偏差为 １８５％ ～３１６％ 。方法操作简便。
A method for determining glycyrrhetic acid in toothpaste by RP\|HPLC is described. The operating conditions were YWG\|C 18 column (4.0 mm i.d.×250 mm, 10 μm)at room temperature, V(methanol)∶V(0.01 mol/L KH 2PO 4)=85∶15 (pH 3.0)as mobile phase with flow rate of 1.0 mL/min and UV detection at 254 nm. Glycyrrhetic acid in toothpaste samples were extracted with methanol. The average recoveries of glycyrrhetic acid were 99.61% 101.67%. The relative standard deviations were 1.85% 3.16%. The method is simple, rapid and accurate.
出处
《色谱》
CAS
CSCD
北大核心
1999年第5期493-494,共2页
Chinese Journal of Chromatography
关键词
反相
高效液相色谱
甘草次酸
牙膏
测定
reversed\|phase high performance liquid chromatography, glycyrrhetic acid, toothpaste
