摘要
【目的】探讨高效液相色谱（HPLC）法同时测定通塞脉微丸中绿原酸、甘草素、肉桂酸和甘草酸单铵盐含量的方法。【方法】采用Zorbax 80A Extend-C18色谱柱,以乙腈-体积分数0.1%磷酸为流动相,梯度洗脱,二极管阵列检测,测定波长λs=276 nm,参比波长λR=590 nm。【结果】绿原酸在0.050 5-1.616 0μg范围内有良好的线性关系,r=0.999 9。甘草素在0.025 2-0.806 4μg范围内有良好的线性关系,r=0.999 9。肉桂酸在0.011 1-0.355 2μg范围内有良好的线性关系,r=0.999 9。甘草酸单铵盐在0.330 0-10.560 0μg范围内有良好的线性关系,r=0.999 9。平均加样回收率：绿原酸为98.17%,相对标准差（sR）为4.28%（N=6）。甘草素为93.76%,sR为1.94%（N=6）。肉桂酸为98.25%,sR为2.72%（N=6）。甘草酸单铵盐为102.60%,sR为1.93%（N=6）。【结论】该方法操作简便,结果可靠,重现性好,可作为该制剂质量控制的方法。
Objective To establish a HPLC method for the simuhaneous determination of chlorogenic acid, liquiritigenin, cinnamic acid, and monoammonium glycyrrhetate in Tong Saimai Pellets. Methods A Zorbax 80A Extend-C18 column was used. The mobile phase consisted of acetonitrile and 0. 1% phosphoric acid, and was used for gradient elution. Chlorogenic acid, liquiritigenin, cinnamic acid and monoammonium glycyrrhetate were determined by dual wavelength, λs = 276nm, λR = 590nm. Results The linear range of chlorogenic acid was 0. 0505 - 1. 6160 μg, and r =0. 9999; the average recovery was 98.17% , and s, =4. 28% （N =6）. The linear range of liquiritigenin was 0. 0252 - 0. 8064 μg, and r = 0. 9999 ; the average recovery was 93.76% , and sR = 1.94% （N = 6）. The linear range of cinnamic acid was 0.0111 -0. 3552μg, and r = 0.9999; the average recovery was 98.25%, and sR =2.72% （N =6）. The linear range of monoammonium glyeyrrhetate was 0. 3300 -10. 5600μg, and r = 0. 9999 ; the average recovery was 102.6% , and sR = 1.93% （N = 6）. Conclusion This method is convenient, rapid and accurate, and it can be used for quality control of the production of Tong Saimai Pellets.
出处
《广州中医药大学学报》
CAS
2009年第6期554-558,共5页
Journal of Guangzhou University of Traditional Chinese Medicine
基金
国家科技部863计划资助项目(编号:2003AA2Z3247)
江苏省自然科学基金资助项目(编号:BK2001209)
关键词
通塞脉微丸/化学
绿原酸/分析
肉桂酸/分析
甘草素/分析
甘草酸单铵盐/分析
色谱法
高压液相
TONG SAIMAI PELLETS/chemistry
CHLOROGENIC ACID/analysis
CINNAMIC ACID/analysis
LIQUIRITIGENIN/analysis
MONOAMMONIUM GLYCYRRHETATE/analysis
CHROMATOGRAPHY, HIGH PRESSURE LIQUID
