摘要
背景:有报道胶原-氨基聚糖材料基体植入周围神经组织节段性损伤处,可以促进神经轴突修复再生。硫酸肝素是细胞外基质的重要组成部分。目的:制备胶原蛋白-硫酸肝素神经组织工程生物支架,观察其生物相容性并用于神经损伤的修复。方法:将胶原蛋白-硫酸肝素悬浊液冷冻干燥,通过显微物镜、扫描电镜观察内部孔隙结构,测量孔的大小。选取SD大鼠12只制备5mm坐骨神经神经缺损模型,随机分为3组。硫酸肝素复合胶原支架移植桥接组、空白对照组(旷置)、正常对照组(自体神经移植)。移植术后经神经功能学观形态学检测其修复疗效。结果与结论:胶原蛋白复合硫酸肝素支架材料具有纵行的、平行排列的微管结构,高度仿生神经的内部空间三维结构。组织学染色、电镜观察证实再生神经纤维成功地长入组织工程材料内。部分大鼠运动功能恢复良好。结果提示,胶原蛋白复合硫酸肝素材料组织相容性良好,可以促进神经的再生并可用于神经损伤的修复。
BACKGROUND：Collagen-amino polysaccharide material matrix implanted into segmental injury site surrounding nerve tissues,can promote axonal repair and regeneration.Heparin sulfate is an important component of extracellular matrix.OBJECTIVE：To prepare the collagen-heparin sulfate nerve tissue engineered scaffold,and to study the histocompatibility for repairing neural injury.METHODS：Collagen-heparin sulfate turbid liquid was frozen and dried,the inner pore structure was observed with gross observation and scanning electron microscope,the pore size was also measured.Twelve SD rats were used to prepare 5-mm sciatic nerve defect models,which were then divided into three groups：normal control group（autologous nerve transplantation）,blank control group and collagen-heparin sulfate composite scaffold transplantation group.Morphology characteristic was observed after transplantation and the repair effect was determined.RESULTS AND CONCLUSION：Collagen-heparin sulfate scaffolds had longitudinal micro-tubular structure with parallel arrangement,and three-dimensional structure of bionic nerve.Histological staining and electron microscopy showed regenerated nerve fibers successfully grew into the scaffold,some rats＇ motor functions were good.Collagen-heparin sulfate material has well histocompatibility,it may promote neural regeneration and be used for neural injury repair.
出处
《中国组织工程研究与临床康复》
CAS
CSCD
北大核心
2011年第12期2125-2128,共4页
Journal of Clinical Rehabilitative Tissue Engineering Research
基金
辽宁省教育厅基金项目(2008403)
辽宁省教育厅创新团队项目(2006T062)~~
