摘要
目的探讨壳聚糖与琼脂糖水凝胶载体及外源性神经生长因子联合制成组织工程神经支架复合体修复大鼠周围神经缺损的可行性。方法壳聚糖与琼脂糖水凝胶载体及外源性的神经生长因子结合,制备成新型的组织工程神经支架复合体,修复大鼠坐骨神经10mm缺损,术后16周进行免疫组织化学等再生神经形态学观察。用单纯壳聚糖导管和自体神经分别作为阴性、阳性对照。结果复合支架组的神经再生数目和直径均优于单纯壳聚糖导管组(P<0.01),与自体神经移植组相当。结论壳聚糖与琼脂糖水凝胶载体及外源性的神经生长因子结合,制备成的组织工程神经支架复合体对大鼠神经再生提供良好的再生微环境,可明显促进神经再生,效果接近自体神经移植水平。
Objective To study the feasibility to repair the peripheral nerve gap with tissue engineering scaffold complex that is composed of medical biodegradable material agarose hydrogel and nerve growth factor （NGF）. Methods Chitosan tube containing agarose hydrogel and NGF was transplanted to bridge a 10 mm gap of injured sciatic nerve in rat. Chitosan duct without agarose hydrogel and NGF was used as negative control, while autograft nerve as positive control. Sixteen weeks after operation, the regeneration of nerve fiber was observed with morphological and immunohistochemistrical methods. Result The number and diameter of regenerating nerve fibers bridged by the scaffold complex of agarose hydrogel and NGF were better than negative control group （P〈 0.01）and reached the level of autograft nerve group. Conclusions The new type of tissue engineering scaffold complex of agarose hydrogel and NGF may provide a microenvironment, as well as autograft nerve, to promote nerve regeneration. This technique may benefit patients with nerve injury in the future.
出处
《中国医学科学院学报》
CAS
CSCD
北大核心
2005年第6期688-691,i0011,共5页
Acta Academiae Medicinae Sinicae
基金
国家高技术研究发展计划项目(863项目)(2003AA205004
2002BA711A0)
辽宁省教育厅科学基金(202173365)~~
