摘要
观察高脂饲料诱发的C57BL/6J小鼠血脂、转氨酶和肝脏脂质的动态变化。C57BL/6J分成基础饲料组和高脂饲料组,在给予高脂饲料后5、7、9、11周采血检测血脂、转氨酶,并制成10%肝匀浆检测肝脏脂质。取肝脏HE染色、油红O染色作病理学检查。结果表明,给予高脂饲料5周小鼠血脂TC和ALT显著升高,7～9周稳定,11周稍有下降。肝脏显示明显病理学损伤,呈现轻至中度非酒精性脂肪肝。高脂饲料引起C57BL/6J小鼠血脂TC和ALT升高迅速、明显,适合非酒精性脂肪肝模型的制作。
To establish a non-alcoholic steatohepatitis animal model induced by high-fat diet,and observe the dynamic variation of relevant index at different time.C57BL/6J mouse were randomly divided into 2 groups.The control group was fed with standard diet and the model group fed with high-fat diet.The serum TG,TC,ALT,AST,and ALP were assayed at the 5th,7th,9th and 11th week.Some mice were killed and the liver were taken,and liver homogenate lipid were detected.HE-stained sections were prepared for histopathological examination.The levels of TC and ALT in serum rose rapidly after 5 week of high-fat diet,and stayed at a high level throughout the duration of 7-9 weeks,and 11 week decreased a little.Pathological changes were observed in the liver.Animal Models presented slight to moderately degree Non-alcoholic Steatohepatitis.Feeding with high-fat diet could induce hyperlipidemia in mice rapidly and reliably,thus this method fit the establishment of animal model in non-alcoholic steatohepatitis.
出处
《东北农业大学学报》
CAS
CSCD
北大核心
2011年第3期98-104,共7页
Journal of Northeast Agricultural University
基金
国家自然科学基金资助项目(30871891)
