摘要
目的:筛选和鉴定与多囊卵巢综合征(Polycysticovarysyndrome,PCOS)胰岛素抵抗(InsulinresistanceI,R)相关的蛋白。方法:采用固相pH梯度双向凝胶电泳分离血清蛋白质,银染显色,通过凝胶成像仪获取图像,PDQuest软件分析电泳图谱,寻找有意义的差异蛋白点,运用MALDI-TOF-MS质谱鉴定,分析其意义。结果:选取具有明显差异的5个蛋白点进行质谱鉴定,经数据库查询,2个蛋白点获得了有意义的结果,分别为结合珠蛋白(Haptoglo-bin,HP),RhoA蛋白。结论:PCOSIR患者与非胰岛素抵抗(Non-Insulin resistance,non-IR)PCOS妇女血清蛋白表达存在差异,这些差异蛋白有可能是PCOS IR特异相关性蛋白,并有可能成为PCOSIR诊断的分子标志物。
Objective： To find out disease specific-proteins（DSPs） which are closely related with polycystic ovary syndrome（PCOS） and insulin resistance（IR）.Methods： The serum proteins were separated by immobilized pH gradient-based two-dimensional gel eletrophoresis（2-DE）.The gels were stained by silver,scanned by gel formatter,and analyzed by using PDquest software.The differentially displayed protein spots associated with PCOS IR were searched for and identified by MALDI-T0F-MS.Results： Five DSPs were chosen for test and two DSPs were found,which were haptoglobin and RhoA protein.Conclusion：There are different protein expressions in 2D-PAGE of PCOS IR and non-IR PCOS.These proteins may be DSPs of PCOS IR,and may be the markers for diagnosis.
出处
《重庆医科大学学报》
CAS
CSCD
北大核心
2011年第2期145-147,共3页
Journal of Chongqing Medical University
基金
重庆市卫生局基金项目(编号:2007-2-25)
关键词
多囊卵巢综合征
胰岛素抵抗
血清
蛋白
polycystic ovary syndrome
insulin resistance
serum
protein
