摘要
目的:观察肝必康胶囊体内抗鸭乙型肝炎病毒(DHBV)的作用。方法:采用广西麻鸭乙型肝炎动物模型,用肝必康胶囊灌胃,治疗1周(T7)、2周(T14)、停药3天(P3)时检测用药前后血清中的DHB-VDNA、DHBsAg、DHBeAg及血清转氨酶(ALT、AST),并取肝组织进行HE染色,光镜观察病理学变化。结果:与模型对照组比较,肝必康大、中剂量用药2周(T14)后能使血清中DHBVDNA含量总体水平显著降低(P<0.05),用药1周后鸭血清HBsAg、HBeAg滴度开始下降,肝必康大、中剂量组有显著性差异(P<0.01)。肝必康胶囊用药后血清中ALT及AST活性有明显下降趋势(P<0.01)。病理学观察表明肝必康胶囊对肝组织病理变化有明显改善作用。结论:肝必康胶囊连续用药2周具有抗鸭乙肝病毒、降低转氨酶活性及改善鸭肝脏炎性病变作用。
Objective:To observe the inhibitory effect of ganbikang capsule on duck hepatitis B in vivo.Methods:60ducks were divided into five groups,which were injected duck serum with positive DHBV DNA to make models except normal group.Each group was given the corresponding medicine,serum DHBV DNA was determined by real time quantitative PCR after treated 7,14 days and stopping taking medicine for 3 days,meanwhile determined serum DHBsAg and DHBeAg by ELISA method and serum ALT and AST levels,taking ducks' hepatic tissue to observe the pathologic changes by HE staining and light scope.Results:To compare with model group,the total content of serum DHBV DNA was lowered obviously in the high and middle dose ganbikang capsule groups after treated 2weeks,the levels of ducks' serum HBsAg and HBeAg in the two ganbikang groups began to lower after treated 1 week,there were significant difference between the two ganbikang groups and model group.The activities of ducks' serum ALT and AST were obviously reduced after treated with ganbikang capsule.Ganbikang capsule could significantly improve the pathologic changes of ducks' livers.Conclusion:Ganbikang can inhibit duck hepatitis B and protect hepatic function.
出处
《中国中医药科技》
CAS
2011年第2期97-99,87,共3页
Chinese Journal of Traditional Medical Science and Technology
基金
广西科技厅科技攻关资助项目No.桂科攻(0235023-3)
