摘要
目的:观察复方六月雪(六月雪、白花蛇舌草、栀子根等)体外抗HBV的作用。方法:采用血清药理学法进行实验,复方六月雪含药血清作用于HepG2.2.15细胞,分别在第72h和144h收集细胞培养上清液,采用ELISA法测定上清液HBsAg和HBeAg的滴度。结果:复方六月雪含药血清在HepG2.2.15细胞培养中可有效地抑制细胞HBsAg和HBeAg的表达(P<0.05、P<0.01),抑制作用有明显的量效反应关系。结论:复方六月雪含药血清在体外具有显著的抗HBV作用。
AIM： To study the inhibitory effect of Compound Liuyuexue （ Tarphochlamys offinis （Gift） Bremekhu, Herba Hedyotis, Raidx Gardeniae, etc. ） （CLYX） on HBV in vitro. METHODS： The serum containing CLYX was added to cultured HepG2.2.15 cells, and HepG2.2.15 cells were cultured in medium containing the serum with CLYX for 72 h and 144 h. The culture media were collected for determining the levels of HBsAg and HBeAg by ELISA. RESULTS： The serum containing CLYX could markedly inhibit HBsAg and HBeAg expressions in the HepG2.2.15 cells （P 〈0.05, P 〈0.01 ）. The anti-HBV effect of CLYX was dose-dependent. CONCLUSION： CLYX can significantly inhibit HBV in vitro.
出处
《中成药》
CAS
CSCD
北大核心
2007年第6期805-808,共4页
Chinese Traditional Patent Medicine
基金
广西科学研究与技术开发计划项目(桂科攻0322024-5E)
