摘要
采用光的矢量衍射理论,研究了0/π圆形相位板不同设计参数对于受激发射损耗(STED)显微镜中抑制光形成中空聚焦光斑聚焦质量的影响,并进行了数值模拟。相关计算结果表明,当采用不同偏振态、不同光强分布的入射光作为STED光,0/π圆形相位板内径的合理取值范围是不同的,而系统所用物镜的数值孔径对于内径的取值影响较小。在各种不同的参数组合下,使用线偏振光和圆偏振光作为入射光时,0/π圆形相位板内径取值范围基本保持一致;当入射光为径向偏振光时,内径取值则相对较大;而当使用切向偏振光时,任何取值均无法在系统焦平面附近得到满意的中空聚焦光斑。
By use of vectorial diffraction theory,intensity distribution around focal spot of stimulated emission depletion（STED） beam in STED microscopy is numerically calculated and influence of diverse 0/π circular phase plate to performance of focal spot is evaluated.It is demonstrated that dark spot in center of focal plane can never be expected unless proper parameter of phase plate is selected,while such parameters are diverse with various polarizations and beam shapes of incident beams.Furthermore,effect of numerical aperture is comparatively neglectable.Explicit calculation further presents that reasonable value ranges of the inner radii of the phase plate are almost the same if the STED beam is linearly or circularly polarized,which is smaller than that of system with a radially polarized incidenct beam and the beam with azimuthal polarization can never be the proper option for STED beam.
出处
《光学学报》
EI
CAS
CSCD
北大核心
2011年第3期206-209,共4页
Acta Optica Sinica
基金
浙江省自然基金(Y1100408)
中央高校基本科研业务费专项资金(2010QNA5035)
现代光学仪器国家重点实验室基金资助课题
关键词
受激发射损耗显微技术
相位板
衍射理论
数值孔径
stimulated emission depletion（STED） microscopy
phase plate
diffraction theory
numerical aperture
