摘要
目的 研究活化的Cdc42相关激酶1（ACK1）在食管鳞癌组织中的表达水平及其临床意义.方法 回顾性收集1990至2001年间在中国医学科学院肿瘤医院接受手术治疗的原发食管癌病例120例,可分析病例为105例.Western印迹检测食管鳞癌细胞中ACK1的表达.免疫组织化学检测了组织芯片中105例和普通病理切片中45例食管鳞癌及其配对的癌旁正常食管黏膜中ACK1蛋白表达水平.半定量RT-PCR检测了45对冰冻食管鳞癌组织中ACK1的mRNA表达水平.结果 组织芯片中,ACK1在食管正常黏膜中表达弱阳性,与正常黏膜相比,48.6%的肿瘤组织中表达明显上调.ACK1表达升高与食管鳞癌患者淋巴结转移和 TNM分期相关.普通病理切片的结果与组织芯片结果一致.ACK1高表达与患者预后差之间具有显著相关性（P=0.030）.ACK1的mRNA水平在食管鳞癌组织中表达升高,与淋巴结转移和TNM分期相关,与ACK1蛋白水平之间具有相关性（P=0.021）.结论 ACK1在食管鳞癌中表达水平升高,促进食管鳞癌的进展影响患者的预后.
Objective To investigate the expression and relationship of activated Cdc42-associated kinase 1 （ ACK1 ） and the clinical characteristics of esophageal squamous cell carcinoma （ESCC）. Methods The ACK1 expression in ESCC cell lines was detected by Western blot Immunohistochemistry was performed to assay the expression level of ACK1 protein in tumor tissues and adjacent normal epithelium from 105 ESCC patients in tissue microarray and 45 patients in normal tissue slices. Semi-quantitative RT-PCR （reverse transcription-polymerase chain reaction）was performed to determine the expression level of ACK1 mRNA in 45 pairs of ESCC frozen tissues. Results The expression level of ACK1 protein was significantly upregulated in 48. 6% ESCC tissues as compared with the normal adjacent epithelium in tissue microarray. The overexpression of ACK1 was significantly correlated with the lymph node metastasis and TNM stage of ESCC patients. The results of normal tissue slices were consistent with those of tissue microarray. Furthermore the overexpression of ACK1 was associated with a poor survival of ESCC patients （ P = 0. 030 ）. The elevated mRNA level of ACK1 in ESCC tissues was correlated with the lymph node metastasis and TNM stage of ESCC patients. And a significant correlation was observed between protein and mRNA level of ACK1 （P =0.021 ）.Conclusion The up-regulated expressions of ACK1 protein and mRNA are correlated with the progression and prognosis of ESCC.
出处
《中华医学杂志》
CAS
CSCD
北大核心
2011年第3期166-170,共5页
National Medical Journal of China
基金
国家＂863＂高技术研究发展计划 （2006AA020707）
