摘要
目的应用双向凝胶电泳试验初步分离淋病奈瑟菌头孢曲松临床敏感株和诱导耐药株的差异蛋白质。方法分别提取两种细菌的总蛋白,进行等电点聚焦和SDS-聚丙烯胺凝胶电泳,对所得胶图利用Im-ageMaster5.0软件进行分析。结果得到了分辨率和重复性均好的双向凝胶电泳图谱,通过分析得到1.3倍差异点24个。结论通过双向凝胶电泳试验,分离出淋病奈瑟菌头孢曲松临床敏感株和诱导耐药株之间的差异表达蛋白。
Objective To separate different proteins between ceftriaxone sensitive and resistant strains of Neisseria gonorrhoeae by two-dimensional polyacrylamide gel electrophoresis（2-DE）. Methods After preparation of the total proteins,solubilized proteins were separated in the first molecular in IPG （immobilized pH gradient） strips depending on their pI and subsequently in the second dimension according to their molecular weight by SDS-PAGE. Those differential protein spots were visualized by image analysis software of ImageMaster 5.0. Results The repeatability and resolution of Gel electrophoresis were high. 2-DE was performed to find 24 expression-altered proteins （1.3 fold） between susceptible and resistant strains. Conclusion There are differential proteins between ceftriaxone susceptible and resistant strains of Neisseria gonorrhoeae,which can be effectively separated by optimizing the 2-DE.
出处
《中国皮肤性病学杂志》
CAS
北大核心
2010年第12期1097-1099,1110,共4页
The Chinese Journal of Dermatovenereology
