摘要
目的 了解南京地区四环素高度耐药淋球菌（TRNG）tetM基因的分子流行病学情况。方法 采用琼脂稀释法检测菌株是否为TRNG，采用纸片酸度定量法测定菌株是否产β-内酰胺酶。采用单管PCR方法对TRNG阳性菌株作terM基因分型。结果 1999-2004年间南京地区804株淋球菌中共检出136株（16．92％）TRNG，β-内酰胺酶阳性的淋球菌（PPNG）为290株，所有TRNG中69．85％（95／136）为PPNG／TRNG，TRNG的阳性率逐年增高。tetM基因分型结果显示，荷兰变型为135株，美国变型仅1株。结论 南京地区流行的TRNG以荷兰型tetM基因为主（99．26％），美国型仅为偶发。
Objective To investigate the molecular epidemiology of tetM gene in Neisseria gonorrhoeae strains with high level resistance to tetracycline （ TRNG ） in Nanjing, Methods TRNG were detected by an agar dilution method. β-1actamase production was examined by paper acidometric testing. A single tube PCR was used to subtype tetM gene in TRNG. Results The isolation rate of TRNG increased year by year from 1999 to 2004, and a total of 136 （ 16.92% ） TRNG sWains were isolated out of 804 Neisseria gohorrhoeae sWains in Nanjing during this period. Of these TRNG, 69.85% （ 95/136 ） produced β-lactamase （ PPNG/TRNG ）. Subtyping of tetM gene showed that 135 TRNG were Dutch variant, while only one was American variant. Conclusion In Nanjing, Dutch variant is the predominant tetM determinant in TRNG, whereas American variant is the sporadic determinant.
出处
《中华皮肤科杂志》
CAS
CSCD
北大核心
2007年第3期133-135,共3页
Chinese Journal of Dermatology
