摘要
目的动态观察益气活血药对心梗心肌缺血模型大鼠血清TNF-α、IL-6变化的影响。方法采用结扎SD大鼠左冠状动脉前降支方法制备动物模型,在心电图评价的基础上,将造模成功的动物随机分为模型组、益气药组、活血药组、益气活血1∶2组、益气活血2∶1组和通心络组,以假手术组和空白组为对照,共8组。在术前,术后即刻,给药第7、14和28天,检测血清TNF-α和IL-6变化情况。结果假手术组和空白组指标变化无差异(P>0.05);模型组大鼠在心肌缺血后不同时间点的血清TNF-α、IL-6水平均明显升高(P<0.01),益气活血1∶2组、益气活血2∶1组和通心络组均降低,与模型组比较有非常显著性差异(P<0.05或P<0.01),其中益气活血2∶1组作用显著。结论心梗心肌缺血后炎性细胞因子明显升高,益气活血药有显著的降低作用,其作用效果与益气活血药的配伍剂量有相关性。
Objective To observe the influences dynamically of Chinese medicinal with the actions of supplementing qi and activating blood circulation on serum changes of tumor necrosis factor-α（TNF-α） and interleukin-6（IL-6） in rats with myocardial ischemia induced by myocardial infarction.Methods The rat model was established by ligaturing the left anterior descending coronary artery.On the base of ECG evaluation all successfully modeled rats were randomly divided into the model group,group treated with supplementing-qi Chinese medicinal（supplementing qi group）,group treated with activating-blood-circulation Chinese medicinal（activating blood group）,group treated with 1 fraction of supplementing-qi Chinese medicinal and 2 fractions of activating-blood-circulation Chinese medicinal（1∶2 group）,group treated with 2 fractions of supplementing-qi Chinese medicinal and 1 fraction of activating-blood-circulation Chinese medicinal（2∶1 group）,and group treated with Tongxinluo Capsules（Tongxinluo group）.The sham-operation group and blank group were taken as the control.There were totally 8 groups.The changes of serum TNF-α and IL-6 were observed before the operation,at the moment of operation ended and on the 7th,14th and 28th day after medicinal administration.Results The changes of the indexes had no difference in the sham-operation group and blank group（P0.05）.The serum levels of TNF-α and IL-6 increased significantly in the model group at different time points after myocardial ischemia（P0.01）.The serum levels of TNF-α and IL-6 decreased in the 1∶2 group,2∶1 group and Tongxinluo group,especially in the 2∶1 group,with a greatly significant difference compared with the model group（P0.05 or P0.01）.Conclusion After myocardial ischemia induced by myocardial infarction,inflammatory cytokines increase significantly.The Chinese medicinal with the actions of supplementing qi and activating blood circulation can reduce them significantly.The effect is related to the doses of Chinese medicinal combination.
出处
《北京中医药大学学报》
CAS
CSCD
北大核心
2010年第9期614-617,631,共5页
Journal of Beijing University of Traditional Chinese Medicine
基金
国家自然科学基金项目(No.30772850)
关键词
心肌缺血
TNF-Α
IL-6
益气活血药
大鼠
myocardial ischemia
tumor necrosis factor-α
interleukin-6
Chinese medicinal with the actions of supplementing qi and activating blood circulation
rats
