摘要
目的探讨现代分析技术在中药毒理学研究中的地位和作用。方法对国内近十几年来相关文献进行整理、分析与归纳。结果紫外分光光度法(UV)、原子吸收光谱法(AAS)、荧光光谱法、高效液相色谱法(HPLC)、气相色谱法(GC)、高效液相色谱-质谱联用技术(HPLC-MS)、气相色谱-质谱联用技术(GC-MS)和分子生物学技术是目前中药毒理学研究中常用的分析技术,其中HPLC、GC-MS、HPLC-MS应用最广泛。结论现代分析技术在中药体内过程研究中发挥着重要作用,主要用于血液或肝、肾等重要组织中有毒物质的浓度测定;而且随着现代分析技术的发展和分析仪器的灵敏度的提高,必将促进中药毒理学的进一步发展。随着现代毒理学中新技术、新理论的建立与发现,现代分析技术必将在中药毒效动力学(TD)和毒代动力学(TK)研究中占据不可或缺的地位,发挥着不可替代的作用。
Objective To approach the status and roles of modern analytical techniques in researching for toxicology of Traditional Chinese Medicine. Methods Collect, and summarize domestic related literature of the last decade. Results Ultraviolet Spectrophotometry（UV）, Atomic Absorption Spectrometry（AAS）, Fluorescence Spectroscopy（FS）, High Performance Liquid Chromatography（HPLC）, Gas Chromatography（GC）, High Performance Liquid Chromatography-Mass Spectrometry（HPLC-MS）, Gas Chromatography -Mass Spectrometry（GC-MS） and Molecular Biology Techniques（MBT） are commonly used in toxicology of Traditional Chinese Medicine sdudies, in which HPLC, GC-MS, HPLC-MS are the most widely used. Conclusion Modern analytical techniques in the study of vivo process of Traditional Chin-ese Medicine plays an important role, mainly for the determination of the concentration of toxic substances in blood or liver, kidney and other important tissue; And with the developmentof modern analytical techniques and the improvement of the sensitivity of analytical instruments, they will promote the further development of toxicology of Traditional Chinese Medicine. With the establishment and discoveries of new theories and new technology of modern toxicology, modern analytical techniques will play an more important role in Toxicodynamics （TD） and Toxicokinetics（TK） studies of Traditional Chinese Medicine, and plays an irreplaceable effect.
出处
《中国药物警戒》
2010年第10期613-616,共4页
Chinese Journal of Pharmacovigilance
基金
国家重点基础研究发展计划(973)中医基础理论专项资助项目(2009CB522802)
国家自然科学基金项目(30672649)
山东省科技平台建设项目(2008GG2NS02021)
山东省科技公关关键技术研究课题(2007GG2NS02073)
山东省国际合作引智项目(L20083700336)
关键词
现代分析技术
中药毒理学
modern analytical techniques
toxicology of Traditional Chinese Medicine
