摘要
目的：研究苦参素对成纤维细胞增殖及Ⅲ型原胶原ｍＲＮＡ表达的影响，探讨其抗纤维化的作用机制。方法：用ＭＴＴ法及Ｎｏｒｔｈｅｒｎ杂交分别检测ＮＩＨ３Ｔ３成纤维细胞增殖及Ⅲ型原胶原ｍＲＮＡ的表达。结果：苦参素能明显抑制成纤维细胞增殖及Ⅲ型原胶原ｍＲＮＡ的表达，并呈剂量依赖性。结论：苦参素可通过抑制成纤维细胞增殖及Ⅲ型原胶原ｍＲＮＡ的表达而起到抗肝纤维化作用。
Objective: To study the effect of oxymatrine on fibroblastic proliferation and procollagen mRNA expression in search of effective medicine for liver fibrosis. Methods : The proliferation and procollagen mRNA expression of fibroblast cell was studied with MTT bioassay and Northern blot hybridization. Results: The oxymatrine significantly inhibited fibroblastic proliferation and expression of type procollagen mRNA. Conclusion: These findings suggest that the oxymatrine may be an effective natural medicine in the prevention of liver fibrosis.
出处
《第二军医大学学报》
CAS
CSCD
北大核心
1999年第6期356-358,共3页
Academic Journal of Second Military Medical University
基金
国家自然科学基金
