摘要
目的探讨苦参碱(matrine,Ma)和奥沙利铂(oxaliplatin,L-OHP)联合应用对人结肠癌细胞SW1116的增殖抑制作用及机制。方法用不同浓度的Ma、L-OHP和两药联用处理人结肠癌细胞SW1116,采用MTT法测定细胞的增殖抑制作用,TUNEL法检测细胞凋亡率,半定量RT-PCR方法检测fas和fasL基因mRNA表达水平。结果Ma、L-OHP单独应用和联合应用均能抑制SW1116增殖,Ma和L-OHP联合应用后对人结肠癌SW1116细胞的抑制率明显大于单药,并有显著的协同杀伤作用。与单药相比,联合用药组的细胞凋亡率显著上升(P<0.01),Fas mRNA表达显著上升(P<0.01),FasL mRNA表达明显下降(P<0.01)。结论Ma和L-OHP联合应用具有明显的协同抗结肠癌SW1116细胞增殖的作用,并能诱导细胞凋亡,其机制可能与上调fas基因和下调FasL基因表达有关。
ObjectiveTo investigate the inhibitory effects and mechanism of Matrine combined with Oxaliplatin on human colon cancer cell line SW1116. MethodsThe inhibitory effects of Matrine and Oxaliplatin with various concentrations on the human colon cancer SW1116 cells in vitro were evaluated by using MTT method. Apoptotic rate was determined by TUNEL method. The expression of fas and fasL mRNA were detected by semi-quantitive RT-PCR. ResultsMatrine and Oxaliplatin used alone or combined use could inhibit the proli...
出处
《中国现代医学杂志》
CAS
CSCD
北大核心
2008年第23期3472-3475,共4页
China Journal of Modern Medicine
基金
广西自然基金项目(No:0728248)
广西教育厅科研项目(No:200510197)
右江民族医学院科研项目(No:2004864)
