摘要
目的:研究高脂饲粮条件下,添加不同剂量硫辛酸(LA)对小鼠肠道自由基及微生物菌群的影响。方法:将40只昆明种雄性小鼠随机分成4组,设置正常组、高脂组(16·1%猪油),高脂饲粮中分别添加0·05%和0·1%LA每组,饲喂六周,观察对小鼠血浆组织自由基、抗氧化能力及肠道乳杆菌、大肠杆菌数量的影响。结果:高脂饲料可导致小鼠氧化应激,显著升高血浆、肠道组织自由基水平,降低总抗氧化能力,并降低肠道内乳杆菌数量,增加大肠杆菌数量(P<0·05);而高脂添加LA能显著降低小鼠血浆和肠道自由基水平及MDA含量(P<0·05),提高肠组织SOD活性和肠道与血浆总抗氧化能力(P<0·05),达到接近正常组水平。高脂日粮添加LA使肠道乳杆菌数量显著高于高脂组(P<0·05),大肠杆菌数量显著降低(P<0·05),并与LA添加量呈显著量效关系(P<0·05)。结论:小鼠消化道氧化应激引起肠道微生物区系的紊乱,调整肠道氧化还原状态能够使微生物区系恢复正常。
Objective：To evaluate the effects of lipoic acid（LA）on free radicals and microorganism in mice fed with high-fat diet.Method：Forty mice were randomLy divided into 4 groups：control group,high-fat group（16.1%lard）,high fat plus 0.05% LA group,high fat plus 0.1% LA group.Then the levels of free radicals,intestinal microorganism,MDA,T-AOC and the activities of SOD in the intestinal tract were detected at the end of 6th weak.Results：High-fat diet could increase the level of free radicals,induce oxidative stress in mice and decrease the level of lactobacilli,increase Escherichia coli in the intestinal tract of mice（P0.05）.Addition of LA in high-fat diets could decrease the level of free radicals and MDA of plasma and intestinal tract in mice significantly（P0.05）,enhance the activities of SOD and the level of T-AOC in plasma and intestinal tract（P0.05）,increase the level of lactobacilli and decrease Escherichia coli in the intestinal tract of mice（P0.05）.Moreover,they are linear with the dosage of LA in the diets.Conclusion：Oxidative stress disturbs the balance ofintestinal tract colonies of mice.Good oxidoreduction condition can accommodate the quantity ofintestinal tract colonies of mice fed high-fat.
出处
《食品工业科技》
CAS
CSCD
北大核心
2010年第9期321-324,369,共5页
Science and Technology of Food Industry
基金
国家自然科学基金(30671525,30571347)
关键词
高脂
硫辛酸
自由基
肠道微生物
high-fat diet
lipoic acid
free radical
intestinal tract colonies
