摘要
研究CGMP对健康小鼠盲肠中微生物区系的影响。选取126只BALB/c小鼠,随机分成六组,对照1组(日食组)既不灌胃CGMP也不灌胃安慰剂,对照2组灌胃生理盐水(0.2ml),低剂量组、中剂量组、高剂量组分别灌胃等体积(0.2ml)不同浓度的CGMP溶液,灌胃周期为15d。分别于灌胃前以及实验的3、5、7、10、15d和停止灌胃后1w(即第22d)无菌采集小鼠盲肠内容物进行菌群测定。结果显示,与对照组相比中剂量组可明显促进小鼠肠道中的双歧杆菌(p<0.05)和乳酸杆菌增殖(p<0.01),使肠杆菌、肠球菌和致病菌数量明显减少(p<0.05)。结果表明,合适剂量(100μg/d)的CGMP可有效地促进小鼠盲肠内乳酸杆菌和双歧杆菌的增殖,并显著地抑制相应条件的致病菌的生长。
This study aimed to investigate the effects of CGMP with different doses on microflora in caecum of mice.126 BALB/c mices were randomly divided into six groups （control groups and treatment groups）. The control 1 group was administered only with normal fodder. The control 2 group was administered physiological saline at the dose of 0.2 ml/d. The treatment groups were administered the equal volume of CGMP with different concentrations for 15d. At 0 （before terminating）, 3, 5, 7, 10, 15 and 22 d （after terminating CGMP for 1 week） the microflora in the caecum of the tested mice was analyzed respectively in each group. The results showed that in the middle dose group （100ug/d）, the numbers ofBifidobacterium （p〈0.05） and LactobaciUus increase （p〈0.01）, but those of Enterobacter, Enterococcus, opportunistic pathogenesis decrease （p 〈0.05）, compared with the controls groups. The results prove that appropriate dose of CGMP has the function of regulating the intestinal flora, stimulating Bifidobacterium and Lactobacillus to grow, and inhibiting the growth of pathogenic bacteria.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2008年第10期582-585,共4页
Food Science
