摘要
目的:探讨NO炎症途径在早期膝关节骨性关节炎中的作用及透骨消痛颗粒对膝骨性关节炎早期NO炎症途径的影响。方法:用改良的Hulth法建立骨关节炎动物模型,随机分为6组,模型组、对照组(壮骨关节丸组)、实验Ⅰ组(透骨消痛颗粒低剂量组)、实验Ⅱ组(透骨消痛颗粒中剂量组)、实验Ⅲ组(透骨消痛颗粒高剂量组)及正常组,4周后行关节腔大体观察、关节软骨和滑模组织学观察、关节滑液NO、NOS活性检测、软骨和滑膜组织中iNOS含量测定。结果:骨性关节炎关节局部表达NO。透骨消痛颗粒具有延缓关节大体形态,透骨消痛颗粒可降低关节液NO、NOS含量,降低关节软骨及滑模iNOS的表达。其中透骨消痛颗粒中剂量组及高剂量组作用更明显,但两者之间比较差异无统计学意义。结论:关节局部NO是引起膝骨性关节炎的重要介质;透骨消痛颗粒可抑制NO炎症途径,缓解软骨细胞的退变和软骨基质的降解,有效改善膝骨性关节炎患者的临床症状。
Objective：To observe the effect of nitric oxide （NO） inflammation pathway on primary knee osteoarthritis （KOA） and the effect of Tougu Xiaotong Granule on the KOA. Methods：KOA models by modified Hulth method were randomly divided into 6 groups,model group,control group （Zhuangguguanjie Pill group）,experimental Ⅰgroup （Tougu Xiaotong Granule low dosage）,experimental Ⅱ group （Tougu Xiaotong Granule medium--dose group）,experimental Ⅲ group （Tougu Xiaotong Granule high-dose group） and normal group. Four weeks later,general observation of articular cavity as well as histological observation of synovium and cartilage was performed. Meanwhile,NO and NOS activity test of the articular fluid,and iNOS mRNA test of synovium and cartilage were done. Results：There was NO expression in the local joint of OA. Tougu Xiaotong Granule delayed cataplasis of the articular macroscopy appearance,decrease the level of NO,NOS and the expression of iNOS of synovium and cartilage. The best effective was presented in Tougu Xiaotong Granule medium and high dose groups,but there was no significant difference between the two groups. Conclusion：Local NO may play important roles in the development of KOA. Tougu Xiaotong Granule can inhibit NO inflammation pathway,reduce the apoptosis of cartilage cells and effectively improve the symptoms of KOA as well as promote the knee joint recovery.
出处
《中国中医骨伤科杂志》
CAS
2010年第8期3-5,共3页
Chinese Journal of Traditional Medical Traumatology & Orthopedics
基金
福建省青年人才项目(编号:2007F3121)
福建省中西医结合老年性疾病重点实验室开放课题
陈可冀中西医结合发展基金资助(编:CKJ20080572008J1004-20)
关键词
透骨消痛颗粒
骨性关节炎
NO炎症途径
Tougu Xiaotong Granule
Osteoarthritis
NO inflammation pathway
